FLORIDA COOPERATIVE FISH AND WILDLIFE RESEARCH UNIT
FLORIDA COOPERATIVE FISH AND WILDLIFE RESEARCH UNIT
复制标题
佛罗里达鱼类和野生动物合作研究单位
DOI：
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
M. Allen
中科院分区：
文献类型：
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作者：
M. Allen
15.可交付成果的报告期：最终报告截止日期：2003年12月31日项目摘要（最多4000个字符）：LETV抗原LETV表达肽将用于开发LETV ELISA，遵循先前使用完整LET疱疹病毒建立的ELISA方法。有了表达的抗原，可以筛选的血浆样本数量限制将减少。将使用先前通过ELISA使用全病毒检测的血浆样本验证试验的专属性。具体而言，将使用在用BLETV免疫之前和之后从圈养海龟获得的血浆样品。来自人工饲养的绿色海龟的血浆样本（未暴露于疱疹病毒）将作为额外的已知阴性样本。
15. REPORTING PERIOD FOR DELIVERABLES: Final report due: 12-31-03 9. ABSTRACT OF PROJECT(maximum 4000 characters): LETV Antigenic LETV expressed peptides will be used to develop an LETV ELISA following methods previously established for the ELISA using whole LET herpesvirus. With expressed antigen, there will be fewer limitations on the number ofplasma samples that can be screened. Plasma samples previously tested by the ELISA using whole virus will be used to validate the specificity of the assay. Specifically, the plasma samples obtained from the captive-reared turtles prior to and after immunization with inactivate LETV will be used. Plasma samples from captive-reared green turtles with no known exposure to herpesviruses will serve as additional known-negative samples.