Investigating the presence of mealybug species as vectors for viruses in grape-growing areas in Turkey
Investigating the presence of mealybug species as vectors for viruses in grape-growing areas in Turkey
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DOI：
10.1080/07060661.2012.670138
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发表时间：
2012-01-01
影响因子：
2
通讯作者：
Ben-Dov, Yair
中科院分区：
文献类型：
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作者：
Buzkan, Nihal;Karadag, Serpil;Ben-Dov, Yair
2006-2007年7月至8月对土耳其葡萄园进行了调查，以确定是否存在粉蚧侵染以及卷叶病和皱纹木病的病毒因子，即葡萄病毒和Ampeloviruses。收集主要葡萄种植区的植物和昆虫样品并进行处理以进行核酸分离。采用逆转录聚合酶链反应（RT-PCR）技术检测了葡萄藤病毒属（Vitivirus）的葡萄病毒A（GVA）和葡萄病毒B（GVB）以及葡萄卷叶病毒属（Ampelovirus）的葡萄卷叶病毒1型（GLRaV-1）和葡萄卷叶病毒3型（GLRaV-3）。在样品中检测到除GVB外的所有病毒。Planococcus ficus（Signoret）是唯一一种在采样地区广泛分布的粉蚧。这是土耳其首次对这些病毒及其潜在媒介进行流行病学研究。
Vineyards in Turkey were surveyed from July-August of 2006-2007 for the presence of mealybug infestation and the viral agents of leafroll and rugose wood diseases, namely vitiviruses and ampeloviruses, respectively. Plant and insect samples in the main grape-growing regions were collected and processed for nucleic acid isolation. The reverse transcription polymerase chain reaction (RT-PCR) was used to detect the insect-transmitted Grapevine virus A (GVA) and B (GVB) (Vitivirus) and Grapevine leafroll-associated virus-1 (GLRaV-1) and -3 (GLRaV-3) (Ampelovirus). All viruses except GVB were detected in the samples. Planococcus ficus (Signoret) is the only mealybug species found to be widespread in the sampled areas. This is the first epidemiological study of these viruses and their potential vector in Turkey.