AN IMPROVED IMMUNOFLUORESCENCE TECHNIQUE FOR THE HISTOLOGICAL EXAMINATION OF BLOOD-VESSEL TISSUE
AN IMPROVED IMMUNOFLUORESCENCE TECHNIQUE FOR THE HISTOLOGICAL EXAMINATION OF BLOOD-VESSEL TISSUE
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DOI：
10.1016/s0065-1281(89)80082-0
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发表时间：
1989-01-01
影响因子：
2.5
通讯作者：
STEHBENS, WE
中科院分区：
文献类型：
--
作者：
KITTELBERGER, R;DAVIS, PF;STEHBENS, WE
血管样品中弹性纤维的自身荧光是对fitc偶联抗体特异性荧光的常见干扰。用铬黑T反染后，弹性纤维的黄绿色变成了暗红色，从而把一个令人不安的特征变成了一个有用的参考背景。第二种反染剂，对苯二胺，细胞核呈琥珀色。为了证明这种染色技术的改进，对5只羊不同年龄（5 ~ 99个月）的对照静脉、动脉和实验性动脉瘤的血管样本进行冷冻切片，用抗胶原蛋白III、胶原型IV、层粘连蛋白和氮氮原的抗体进行染色。这些结缔组织成分的具体分布现在可能与弹性纤维和细胞（细胞核）的位置有关。
Autofluorescence of elastic fibres in blood vessel samples is a common interference with the specific fluorescence of FITC-conjugated antibodies. Counterstaining with eriochrome black T changed the yellow-green colour of elastic fibres to dark red, thus turning a disturbing feature into a useful reference background. A second counterstain, p-phenylenediamine, visualized cell nuclei as an amber colour. To demonstrate the improvement of this staining technique, cryosections from blood vessel samples, derived from control veins, arteries and experimental aneurysms of different ages (5 to 99 month old) in 5 sheep, were stained with antibodies against procellagen III, collagen type IV, laminin, and nidogen. The specific distribution of these connective tissue components could now be related to the location of the elastic fibres and the cells (cell nuclei).