CagA and VacA genes of Helicobacter pylori and their clinical relevance
CagA and VacA genes of Helicobacter pylori and their clinical relevance
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DOI：
10.4103/ijpm.ijpm_234_17
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发表时间：
2018-01-01
影响因子：
1
通讯作者：
Swaminathan, Mukundan
中科院分区：
文献类型：
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作者：
Jeyamani, Lavanya;Jayarajan, Jayalakshmi;Swaminathan, Mukundan
内容：幽门螺杆菌与多种胃十二指肠疾病的发生有关，这些疾病因种族和感染人群的菌株类型而异。目的：本研究旨在评估H。pylori cagA和vacA基因型，并确定它们与它们引起的病变严重程度的关系。设置和设计：本研究是一项观察性横断面研究。对象和方法：从165例消化不良患者的胃活检组织中提取DNA。用PCR方法检测血吸虫cagA和vacA（s1，s2，m1，m2）基因。幽门。对内镜检查结果与毒力基因之间的关联进行统计分析。使用的统计分析：Pearson卡方检验和Fischer精确检验。结果：H. pylori感染率为37%，vacA s1 cagA阳性菌株为优势基因型（54.1%）。vacAs 1亚型在所有消化性溃疡（PUD）患者中均存在。整个正常研究组仅具有VacA s2变体。这清楚地表明，vacA s1是一个重要的毒力标志物，携带s1菌株的患者更容易发生溃疡（P = 0.007）。有一个显着的关联cagA与s1株，而不是s2。VacA m基因型的变化似乎与疾病状态没有任何关联。cagA基因的存在与PUD而非非溃疡性消化不良之间存在统计学显著相关（P = 0.027）。结论：在我们的人群中的优势基因型是cagA阳性vacA s1，这被发现是显着相关的胃病患者，特别是PUD。VacA s1可以作为疾病表现的单一最佳毒力标记。
Context: Helicobacter pylori is associated with the development of a variety of gastroduodenal diseases which varies with ethnicity and the type of strains that infect the population. Aims: This study aims to evaluate the prevalence of H. pylori cagA and vacA genotypes in our region and to determine their relationship to the severity of the lesions that they cause. Settings and Design: This study was an observational cross-sectional study. Subjects and Methods: DNA was extracted from 165 gastric biopsies from patients evaluated for dyspepsia. PCR was used to detect cagA and vacA (s1, s2, m1, m2) genes of H. pylori. Statistical analysis of associations was performed between endoscopy findings and virulence genes. Statistical Analysis Used: Pearson Chi-square test and Fischer's exact test. Results: The prevalence of H. pylori infection was 37% and the dominant genotypes was vacA s1 cagA-positive strain (54.1%) in this study. The vacAs1 subtype was found in all patients with peptic ulcer disease (PUD). The entire normal study group had VacA s2 variant only. This clearly shows that vacA s1 is a significant virulence marker and patients harboring s1 strains are more prone to develop ulcers (P = 0.007). There was a significant association of cagA with s1 strain rather than s2. Variation in VacA m genotype did not seem to have any association with disease status. There was a statistically significant association between the presence of cagA gene and PUD rather than the nonulcer dyspepsia (P = 0.027). Conclusion: The predominant genotype in our population was cagA positive vacA s1, which was found to be significantly associated with patients with gastric diseases, especially PUD. VacA s1 can serve as a single best virulence marker of the disease manifestation.