Surface-enhanced Raman scattering studies on the recognition mechanism of glutathione peroxidase mimics, bis-cyclodextrin diselenide
Surface-enhanced Raman scattering studies on the recognition mechanism of glutathione peroxidase mimics, bis-cyclodextrin diselenide
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表面增强拉曼散射研究谷胱甘肽过氧化物酶模拟物双环糊精二硒化物的识别机制
DOI：
10.1016/j.colsurfa.2004.10.035
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发表时间：
2005-05-05
影响因子：
5.2
通讯作者：
Luo, GM
中科院分区：
文献类型：
--
作者：
Hao, YQ;Wu, YQ;Luo, GM
利用表面增强拉曼散射（Sers）技术研究了含硒谷胱甘肽过氧化物酶（GPX）模拟物2，2 '-二硒基-双-β-环糊精（2-SeCD）与谷胱甘肽（GSH）替代物S-二硝基苯基谷胱甘肽（GSH-S-DNP）和4，4'-双（羧亚甲基氧基）偶氮苯（BCMAB）之间的识别机理. Sers光谱已被用来研究这些替代品如何与β-CD和2-SeCD结合。在银胶溶液中，GSH-S-DNP和BCMAB的Sers信号比它们的配合物GSH-S-DNP/CD和BCMAB/CD的SERS信号强得多，说明GSH-S-DNP和BCMAB在与β-CD或2-SeCD结合时被纳入CD空腔。在银胶体溶液中，GSH-S-DNP/CD的Sers光谱中存在微弱的拉曼信号，这给有效地利用Sers研究CD与GSH-S-DNP的识别机理带来了困难。在BCMAB/CD的Sers光谱中在1388和1379 cm（-1）处的带强烈地表明BCMAB不通过其-COOH基团与β-CD或2-SeCD结合。(c)2004 Elsevier B. V.保留所有权利。
Surface-enhanced Raman scattering (SERS) spectroscopy has been used to explore the recognition mechanism between a selenium-containing glutathione peroxidase (GPX) mimic, 2,2'-diseleno-bis-p-cyclodextrin (2-SeCD) and two glutathione (GSH) alternatives, S-dinitrophenyl-glutathione (GSH-S-DNP) and 4,4'-bis(carboxymethyleneoxy)azobenzene (BCMAB). SERS spectra have been employed to investigate how these alternatives bind with beta-CD and 2-SeCD. SERS signals of the spectra of GSH-S-DNP and BCMAB are much intense ill comparison with those of theirs complexes, GSH-S-DNP/CDs and BCMAB/CDs in silver colloid solutions, which suggest that GSH-S-DNP and BCMAB are included into the CD cavity when they bind with beta-CD or 2-SeCD. Weak Raman signals in the SERS spectra of the GSH-S-DNP/CDs in silver colloid solution make it difficult to investigate the recognition mechanism between CDs and GSH-S-DNP by using SERS efficiently. The bands at 1388 and 1379 cm(-1) in the SERS spectra of BCMAB/CDs strongly suggest that BCMAB does not bind with beta-CD or 2-SeCD via its -COOH group. (c) 2004 Elsevier B.V. All rights reserved.