Computer-averaged views of the 70 S monosome from Escherichia coli.
Computer-averaged views of the 70 S monosome from Escherichia coli.
复制标题
大肠杆菌 70 S 单体的计算机平均视图。
DOI：
10.1016/0022-2836(86)90336-0
复制
发表时间：
1986
影响因子：
5.6
通讯作者：
Boublik,M
中科院分区：
文献类型：
--
作者：
Verschoor,A;Frank,J;Wagenknecht,T;Boublik,M
摘要 原核生物 (70 S) 单体由大致半球形的 50 S 大亚基和细长的 30 S 小亚基组成，在电子显微照片中仅以少数常见视图出现，代表了颗粒所呈现的少量首选方向。其中两个被称为 O 和 L 视图，之前被描述为重叠和非重叠投影；第三个视图，我们称之为 R 视图，表示具有重叠或 O 视图的旋转连续体的另一个端点。倾斜研究使我们能够将该范围校准为跨度~ 50°。对 L 视图的分离集进行平均，并将可重复分辨率确定为 1 3.5 nm− 1。通过对应分析对 O 和 R 视图的组合集进行分析，并获得子平均值的连续“旋转系列”。根据对各个亚基形态的了解来解释观点，可以构想出单体中亚基相互配合的一般情况。
Abstract The prokaryotic (70 S) monosome, composed of a roughly hemispherical 50 S large subunit and an elongate 30 S small subunit, appears in the electron micrograph in only a few common views representing the small number of preferred orientations assumed by the particle. Two of these, termed O and L views, have previously been characterized as the overlap and non-overlap projections; a third view, which we term the R view, represents the other endpoint of a rotational continuum with the overlap or O view. Tilt studies enabled us to calibrate this range as spanning~ 50°. The disjunct set of L views was averaged, and the reproducible resolution was determined to be 1 3.5 nm− 1. The combined sets of O and R views were analyzed by correspondence analysis, and a continuous “rotation series” of subaverages was obtained. Interpretation of the views in the light of what is known about the morphologies of the individual subunits allows a general picture of the mutual fit of the subunits in the monosome to be conceived.