Vascular segmental permeabilities at high peak inflation pressure in isolated rat lungs
Vascular segmental permeabilities at high peak inflation pressure in isolated rat lungs
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DOI：
10.1152/ajplung.00488.2001
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发表时间：
2002-12-01
影响因子：
4.9
通讯作者：
Yoshikawa, S
中科院分区：
文献类型：
--
作者：
Parker, JC;Yoshikawa, S
在离体灌流的大鼠肺中测定肺段滤过系数（K-f）对高峰充气压力（PIP）损伤的反应。在基线条件下和使用40-45 cmH 2 O PIP通气后，测量总（K-f，K-t）、动脉（K-f，K-a）和静脉（K-f，K-v）滤过系数。在I区条件下，通过将气道压力增加至25-27 cmH（2）O，测量K-f、K-a和K-f、K-v。然后通过下式计算微血管段K-f（K-f，K-mv）：K-f，K-mv = K-f，K-t = K-f，K-a - K-f，K-v。基线K-f，K-t为0.090 +/- 0.022 ml·min（-1）·cmH（2）O（-1）·100 g（-1），段性分布18%动脉，41%静脉和41%微血管。高PIP损伤后，K-f、K-t增加680%，而K-f、K-a、K-f、K-v和K-f（mv）分别增加398%、589%和975%。用50 μ M氯化钆预处理防止了所有血管段中PIP诱导的K-f的高增加。这些数据意味着较低的水力传导的微血管内皮细胞，由于其大的表面积和钆敏感的高PIP损伤，产生在肺泡和肺泡外血管段。
The response of segmental filtration coefficients (K-f) to high peak inflation pressure (PIP) injury was determined in isolated perfused rat lungs. Total (K-f,K-t), arterial (K-f,K-a), and venous (K-f,K-v) filtration coefficients were measured under baseline conditions and after ventilation with 40-45 cmH(2)O PIP. K-f,K-a and K-f,K-v were measured under zone I conditions by increasing airway pressure to 25-27 cmH(2)O. The microvascular segment K-f (K-f,K- mv) was then calculated by: K-f,K-mv = K-f,K-t = K-f,K-a - K-f,K-v. The baseline K-f,K- t was 0.090 +/- 0.022 ml.min(-1).cmH(2)O(-1).100 g(-1) and segmentally distributed 18% arterial, 41% venous, and 41% microvascular. After high PIP injury, K-f,K- t increased by 680%, whereas K-f,K-a, K-f,K-v, and K-f,(mv) increased by 398, 589, and 975%, respectively. Pretreatment with 50 muM gadolinium chloride prevented the high PIP-induced increase in K-f in all vascular segments. These data imply a lower hydraulic conductance for microvascular endothelium due to its large surface area and a gadolinium-sensitive high-PIP injury, produced in both alveolar and extra-alveolar vessel segments.