Albumin regulates Runx2 and alpha1 (I) collagen mRNA expression in osteoblastic cells: comparison with insulin-like growth factor-I.
Albumin regulates Runx2 and alpha1 (I) collagen mRNA expression in osteoblastic cells: comparison with insulin-like growth factor-I.
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白蛋白调节成骨细胞中 Runx2 和 alpha1 (I) 胶原 mRNA 表达：与胰岛素样生长因子-I 的比较。
DOI：
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发表时间：
2005
影响因子：
5.4
通讯作者：
M. Yamaguchi
中科院分区：
文献类型：
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作者：
K. Ishida;M. Yamaguchi
已显示白蛋白在成骨细胞中表达。研究白蛋白在成骨细胞中的作用。在不含胎牛血清（FBS）的培养基中，在存在或不存在白蛋白（0.5或1.0 mg/ml培养基）或胰岛素样生长因子-I（IGF-I; 10（-9）或10（-8）M）的情况下，将具有亚融合单层的成骨细胞MC 3 T3-E1培养24或48 h。在白蛋白（0.5或1.0 mg/ml）或IGF-I（10（-9）或10（-8）M）存在下培养48 h，成骨细胞数量显著增加。在IGF-I（10（-9）或10（-8）M）的存在下，白蛋白（0.5或1.0 mg/ml）增加细胞数量的作用没有显著调节。白蛋白（1.0mg/ml）可显著增加成骨细胞蛋白质含量。在IGF-I（10（-9）或10（-8）M）的存在下，这种效应没有显著改变。碱性磷酸酶活性在含白蛋白（0.5或1.0 mg/ ml）的培养物中显著降低，而在含IGF-I（10（-9）或10（-8）M）的培养物中显著升高。在白蛋白（0.5或1.0 mg/ ml）的存在下，IGF-I在增加酶活性方面的作用没有显著改变。白蛋白（0.5 mg/ ml）加IGF-I（10（-9）M）增加细胞数的作用在PD 98059存在下完全被阻止，表明白蛋白或IGF-I的作用部分通过促分裂原活化蛋白（MAP）激酶级联介导。使用转录-聚合酶链反应（RT-PCR）分析，成骨细胞中Runx 2（1型）mRNA的表达在白蛋白（0.5或1.0 mg/ml）或IGF-I（10（-9）M）培养24或48 h后显著降低。成骨细胞中α 1（I）胶原mRNA的表达在白蛋白（0.5或1.0 mg/ml）或IGF-I（10（-9）M）培养24 h后显著增加。在小鼠骨髓培养物中，白蛋白（0.5、1.0或2.0 mg/ml）对甲状旁腺激素（PTH; 10（-7）M）诱导的破骨细胞样细胞形成无显著影响。本研究表明，白蛋白在成骨细胞中具有调节Runx 2或α 1（I）胶原mRNA表达的作用，这可能是通过细胞内信号通路介导的。
Albumin has been shown to be expressed in osteoblastic cells. The role of albumin in osteoblastic cells was investigated. Osteoblastic MC3T3-E1 cells with subconfluent monolayers were cultured for 24 or 48 h in a medium without fetal bovine serum (FBS) in the presence or absence of albumin (0.5 or 1.0 mg/ml of medium) or insulin-like growth factor-I (IGF-I; 10(-9) or 10(-8) M). The number of osteoblastic cells was significantly increased by culture for 48 h in the presence of albumin (0.5 or 1.0 mg/ml) or IGF-I (10(-9) or 10(-8) M). The effect of albumin (0.5 or 1.0 mg/ml) in increasing the cell number was not significantly modulated in the presence of IGF-I (10(-9) or 10(-8) M). Protein content in osteoblastic cells was significantly increased by culture with albumin (1.0 mg/ml). This effect was not significantly altered in the presence of IGF-I (10(-9) or 10(-8) M). Alkaline phosphatase activity was significantly decreased by culture with albumin (0.5 or 1.0 mg/ ml), while it was significantly increased in the presence of IGF-I (10(-9) or 10(-8) M). The effect of IGF-I in increasing the enzyme activity was not significantly altered in the presence of albumin (0.5 or 1.0 mg/ ml). The effect of albumin (0.5 mg/ ml) plus IGF-I (10(-9) M) in increasing the cell number was completely prevented in the presence of PD98059, suggesting that the effect of albumin or IGF-I is partly mediated through mitogen-activated protein (MAP) kinase cascade. The expression of Runx2 (type 1) mRNA using transcription-polymerase chain reaction (RT-PCR) analysis in osteoblastic cells was significantly decreased by culture for 24 or 48 h with albumin (0.5 or 1.0 mg/ml) or IGF-I (10(-9) M). alpha1 (I) collagen mRNA expression in osteoblastic cells was significantly increased by culture for 24 h with albumin (0.5 or 1.0 mg/ml) or IGF-I (10(-9) M). Albumin (0.5, 1.0, or 2.0 mg/ml) did not have a significant effect on osteoclast-like cell formation induced by culture with parathyroid hormone (PTH; 10(-7) M) in mouse marrow culture. This study demonstrates that albumin has a role in the regulation of Runx2 or alpha1 (I) collagen mRNA expression, which may be mediated through intracellular signaling pathway, in osteoblastic cells.