The effects of NB-UVB on the hair follicle-derived neural crest stem cells differentiating into melanocyte lineage in vitro
The effects of NB-UVB on the hair follicle-derived neural crest stem cells differentiating into melanocyte lineage in vitro
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NB-UVB对毛囊源性神经嵴干细胞体外分化为黑素细胞谱系的影响。
DOI：
10.1016/j.jdermsci.2012.01.012
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发表时间：
2012-04-01
影响因子：
4.6
通讯作者：
Xiang, Leihong
中科院分区：
文献类型：
--
作者：
Dong, Dake;Jiang, Min;Xiang, Leihong
背景：窄谱中波紫外线（NB-UVB）是治疗白癜风的一种有效的治疗方法。目的：探讨NB-UVB对毛囊源性神经嵴干细胞（HF-NCSCs）体外诱导黑素细胞系成熟的影响。分离的细胞是多能的，并表达胚胎NCSC生物标志物。评价NB-UVB对HF-NCSCs发育和分化的影响。我们评估了NB-UVB辐射后源自HF-NCSC的黑素细胞的细胞活力、黑素生成和迁移。采用定量RT-PCR检测酪氨酸酶、Tyrp 1、Dct、Kit、Mc 1 R、Fzd 4、NT 3R、Ednra、EP 1、TGF β 12、Sox 10、Mitf、Lef 1和Pax 3基因表达，采用Western blot检测酪氨酸酶、Sox 10和Mitf蛋白表达。结果：NB-UVB促进小鼠HF-NCSCs黑素细胞分化过程中酪氨酸酶的表达，但抑制HF-NCSCs黑素细胞的增殖。从机制上讲，NB-UVB治疗后黑素细胞成熟增加是由于几种关键的黑素生成因子表达增加，包括Sox 10，Kit和Mc 1 R，它们对促进酪氨酸酶表达起关键作用。此外，HF-NCSC衍生的黑素细胞的迁移随着NB-UVB剂量的增加而下调。结论：NB-UVB对HF-NCSCs诱导分化的黑素细胞色素沉着有直接影响，为NB-UVB治疗白癜风提供了可能的机制。(C)2012年日本皮肤病研究学会。由Elsevier爱尔兰有限公司出版。保留所有权利。
Background: Narrow-band UVB (NB-UVB) is an effective therapeutic option in the treatment of vitiligo. Despite the apparent clinical efficacy, the underlying mechanism of how topical NB-UVB induces repigmentation in vitiligo has not been clearly elucidated.Objectives: To investigate the effects of NB-UVB on the maturation of melanocyte lineage differentiated from hair follicle-derived neural crest stem cells (HF-NCSCs) in vitro.Methods: HF-NCSCs were isolated from mouse whisker follicles. The isolated cells were multipotent and expressed embryonic NCSC biomarkers. The effects of NB-UVB on development and differentiation of HF-NCSCs were evaluated. We assessed cell viability, melanogenesis and migration of melanocytes derived from HF-NCSCs after NB-UVB radiation. Tyrosinase, Tyrp1, Dct, Kit, Mc1R, Fzd4, NT3R, Ednra, EP1, TGF beta 12, Sox10, Mitf, Lef1 and Pax3 gene expression was measured by quantitative RT-PCR, while Tyrosinase, Sox10 and Mitf protein expression were measured by Western blot analysis. Cell migration was measured by Boyden chamber transwell assay.Results: NB-UVB increased the expression of tyrosinase during melanocytic differentiation from mouse HF-NCSCs, however, NB-UVB inhibited proliferation of melanocytes derived from HF-NCSCs. Mechanistically, increased melanocyte maturation after NB-UVB treatment was resulted from increased expression of several key melanogenic factors, including Sox 10, Kit and Mc1R, which play a critical role to promote tyrosinase expression. Furthermore, the migration of the HF-NCSCs-derived melanocytes was downregulated as NB-UVB doses increased. However, the migration of HF-NCSCs was upregulated under 0.4 J NB-UVB radiation.Conclusions: Those data provide in vitro evidence demonstrating some direct effects of NB-UVB on pigmentation of melanocyte lineage differentiated from HF-NCSCs, and may provide a possible mechanism for the effect of NB-UVB in vitiligo. (C) 2012 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.