Inhibition of Activated Coagulation Factor VII by Normal Human Plasma
Inhibition of Activated Coagulation Factor VII by Normal Human Plasma
复制标题
正常人血浆对活化凝血因子 VII 的抑制
DOI：
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发表时间：
1982
影响因子：
6.7
通讯作者：
L. Tjensvoll
中科院分区：
文献类型：
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作者：
P E Dahl;U. Abildgaard;M. Larsen;L. Tjensvoll
建立了一种含组织凝血活素（Tpl）、纯化凝血因子VII和X以及显色底物S-2222的酶解检测系统。在该测试系统中，抗凝血酶III （AT）的抑制作用约占正常血浆总抑制作用的三分之一。通过添加纯化的AT阻断抗体来阻止AT的这种作用。正常血浆和血清显示近似相似的抑制作用。这种抑制作用可能是针对活化因子VII （fviia）的。在Ultrogel ac34上吸附正常血浆的凝胶过滤显示出三个不同于AT的抑制峰。
Summary An amidolytic assay system with tissue thromboplastin (Tpl), purified coagulation factors VII and X, and the chromogenic substrate S-2222 was developed. Antithrombin III (AT) accounts for about one third of the total inhibition exerted by normal plasma in this test system. This effect of AT was prevented by adding purified AT blocking antibodies. Normal plasma and serum showed approximately similar inhibitory effects. The inhibition was probably directed against activated factor VII (F VIIa). Gel filtration of adsorbed normal plasma on Ultrogel AcA 34 showed three inhibitory peaks which were different from AT.