Stability of small ubiquitin-like modifier (SUMO) proteases OVERLY TOLERANT TO SALT1 and -2 modulates salicylic acid signalling and SUMO1/2 conjugation in Arabidopsis thaliana.
Stability of small ubiquitin-like modifier (SUMO) proteases OVERLY TOLERANT TO SALT1 and -2 modulates salicylic acid signalling and SUMO1/2 conjugation in Arabidopsis thaliana.
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DOI：
10.1093/jxb/erv468
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发表时间：
2016-01
影响因子：
6.9
通讯作者：
Sadanandom A
中科院分区：
文献类型：
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作者：
Bailey M;Srivastava A;Conti L;Nelis S;Zhang C;Florance H;Love A;Milner J;Napier R;Grant M;Sadanandom A
小泛素样修饰蛋白酶1和2（SUMO 1/2）已被连接到水杨酸（SA）介导的拟南芥防御信号的调节。为了确定SUMO蛋白酶OVERLY TELERANT TO SALT 1和-2（OTS 1/2）在防御中的作用，并提供SUMO 1/2介导的SA信号转导调节的见解，我们研究了OTS 1/2突变体中SA介导的防御状态。与野生型（WT）植物相比，ots 1 ots 2双突变体表现出对有毒的假单胞菌和更高水平的SA增强的抗性。此外，ots 1 ots 2突变体除了增强SA响应ICS 1表达以外，还表现出SA生物合成基因ICS 1的上调表达。SA刺激OTS 1/2降解并促进SUMO 1/2缀合物的积累。这些结果表明，OTS 1和-2在SA信号传导中的反馈回路中起作用，并且从头OTS 1/2合成对抗SA促进的降解，调节OTS 1/2的丰度以缓和SA信号传导。SUMO 1/2结合物的积累与SA促进的OTS降解相一致，并且除了在别处报道的抑制作用之外，还可能在SA介导的信号传导中发挥积极作用。
Small ubiquitin-like modifier proteases 1 and 2 (SUMO1/2) have been linked to the regulation of salicylic acid (SA)-mediated defence signalling in Arabidopsis thaliana. In order to define the role of the SUMO proteases OVERLY TOLERANT TO SALT1 and -2 (OTS1/2) in defence and to provide insight into SUMO1/2-mediated regulation of SA signalling, we examined the status of SA-mediated defences in ots1/2 mutants. The ots1 ots2 double mutant displayed enhanced resistance to virulent Pseudomonas syringae and higher levels of SA compared with wild-type (WT) plants. Furthermore, ots1 ots2 mutants exhibited upregulated expression of the SA biosynthesis gene ICS1 in addition to enhanced SA-responsive ICS1 expression beyond that of WT. SA stimulated OTS1/2 degradation and promoted accumulation of SUMO1/2 conjugates. These results indicate that OTS1 and -2 act in a feedback loop in SA signalling and that de novo OTS1/2 synthesis works antagonistically to SA-promoted degradation, adjusting the abundance of OTS1/2 to moderate SA signalling. Accumulation of SUMO1/2 conjugates coincides with SA-promoted OTS degradation and may play a positive role in SA-mediated signalling in addition to its repressive roles reported elsewhere.