Involvement of three annexin genes in the ripening of strawberry fruit regulated by phytohormone and calcium signal transduction
Involvement of three annexin genes in the ripening of strawberry fruit regulated by phytohormone and calcium signal transduction
复制标题
植物激素和钙信号转导调控三个膜联蛋白基因参与草莓果实成熟
DOI：
10.1007/s00299-015-1915-5
复制
发表时间：
2016-04-01
影响因子：
6.2
通讯作者：
Luo, Zisheng
中科院分区：
文献类型：
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作者：
Chen, Jingxin;Mao, Linchun;Luo, Zisheng
三个膜联蛋白基因可能参与了草莓果实的成熟过程。草莓果实成熟过程中，植物激素和钙对三种膜联蛋白基因的表达有调控作用。摘要植物膜联蛋白是一种多功能的膜结合蛋白和Ca2+结合蛋白，参与植物的多种发育过程和胁迫反应。从草莓果实中获得的三个膜联蛋白faann5a、FaAnn5bandFaAnn8cDNA编码约35 kDa的氨基酸序列，包含4个膜联蛋白重复序列、Ca2+结合位点、gtp结合基序、过氧化物酶残基以及色氨酸、精氨酸和半胱氨酸的保守氨基酸残基。在果实发育过程中，3/4R期后faann5b和faann5b转录本水平升高，而faann5b转录本水平下降。膜联蛋白的表达模式提示其在草莓果实发育和成熟过程中的潜在作用。膜联蛋白基因的表达也与激素水平高度相关。外源脱落酸（ABA）增强了faann5和faann8的表达，而外源生长素（IAA）抑制了faann5和faann8的表达。然而，ABA和IAA均促进了aann5b转录本的表达水平，表明可能由于其大家族的多功能，对果实中的膜联蛋白进行了独立调控。膜联蛋白基因对外源ABA和IAA抑制剂的响应证实了膜联蛋白参与植物激素信号转导。此外，钙抑制了FaAnn5s （FaAnn5aandFaAnn5b）的表达，促进了faann8的表达。钙和乙二醇四乙酸（EGTA）对膜联蛋白转录水平的影响证实了钙可能介导激素信号转导通路，这有助于阐明钙在果实成熟中的作用机制。因此，FaAnn5s和FaAnn8可能在下游通过钙信号参与植物激素对草莓果实发育成熟的调控。
Key messageThree annexin genes may be involved in the ripening progress of strawberry fruit. Phytohormones and calcium regulate the expressions of three annexin genes during strawberry fruit ripening.AbstractPlant annexins are multi-functional membrane- and Ca2+-binding proteins that are involved in various developmental progresses and stress responses. Three annexinsFaAnn5a,FaAnn5bandFaAnn8cDNA obtained from strawberry fruit encode amino acid sequences of approximately 35 kDa containing four annexin repeats, Ca2+-binding site, GTP-binding motif, peroxidase residue, and conserved amino acid residues of tryptophan, arginine and cysteine. During fruit development, the transcript levels ofFaAnn5aandFaAnn5bincreased whileFaAnn5bdeclined after 3/4R stage. The expression patterns of annexins suggested their potential roles in strawberry fruit development and ripening. Expressions of annexin genes were also highly correlated with hormone levels. In addition, exogenous abscisic acid (ABA) enhanced the expressions ofFaAnn5aandFaAnn8while exogenous auxin (IAA) retarded it. However, both ABA and IAA promoted the transcript levels ofFaAnn5b, indicating the independent regulation of annexins in fruit likely due to multi-functions of their large family. The responses of annexin genes to exogenous ABA and IAA inhibitors verified the involvement of annexins in plant hormone signaling. Besides, calcium restrained the expressions of FaAnn5s (FaAnn5aandFaAnn5b) but promoted the expression ofFaAnn8. Effects of calcium and ethylene glycol tetraacetic acid (EGTA) on the transcript levels of annexins confirmed that calcium likely mediated hormone signal transduction pathways, which helped to elucidate the mechanism of calcium in fruit ripening. Therefore, FaAnn5s and FaAnn8 might be involved in plant hormones’ regulation in the development and ripening of strawberry fruit through calcium signaling in the downstream.