Genotoxic activity of chlorohydroxyfuranones in the microscale micronucleus test on mouse lymphoma cells and the unscheduled DNA synthesis assay in rat hepatocytes.
Genotoxic activity of chlorohydroxyfuranones in the microscale micronucleus test on mouse lymphoma cells and the unscheduled DNA synthesis assay in rat hepatocytes.
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氯羟基呋喃酮在小鼠淋巴瘤细胞的微型微核测试和大鼠肝细胞中的计划外 DNA 合成测定中的基因毒性活性。
DOI：
10.1093/mutage/14.5.457
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发表时间：
1999
期刊：
影响因子：
2.7
通讯作者：
D. Marzin
中科院分区：
文献类型：
--
作者：
F. L. Curieux;F. Nesslany;T. Munter;L. Kronberg;D. Marzin
氯羟基呋喃酮 (CHF) 是经氯处理的饮用水中发现的致突变消毒副产物。在目前的研究中，四种CHF的遗传毒性：3,4-二氯-5-羟基-2(5H)-呋喃酮(MCA)、3-氯-4-甲基-5-羟基-2(5H)-呋喃酮(MCF)、3-氯-4-(氯甲基)-5-羟基-2(5H)-呋喃酮(CMCF)和测定3-氯-4-(二氯甲基)-5-羟基-2(5H)-呋喃酮(MX)。进行了两项体外测定，即 L5178Y 小鼠淋巴瘤细胞的微型微核测定和 Fisher F344 大鼠肝细胞原代培养物的计划外 DNA 合成测定。所有四种 CHF 在两项检测中均显示出遗传毒性作用。在所使用的两个系统中，CMCF 是遗传毒性最强的化合物，其次分别是 MCA、MX 和 MCF。这项工作是在两项体外遗传毒性测试中首次研究所有四种 CHF 的 DNA 损伤特性。这些新数据扩大了这组化合物引起的遗传损伤的范围。
Chlorohydroxyfuranones (CHFs) are mutagenic disinfection by-products found in chlorine-treated drinking water. In the current study, the genotoxicity of four CHFs, 3,4-dichloro-5-hydroxy-2(5H)-furanone (MCA), 3-chloro-4-methyl-5-hydroxy-2(5H)-furanone (MCF), 3-chloro-4-(chloromethyl)-5-hydroxy-2(5H)-furanone (CMCF) and 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX), was determined. Two in vitro assays, the microscale micronucleus assay on L5178Y mouse lymphoma cells and the unscheduled DNA synthesis assay on a hepatocyte primary culture from Fisher F344 rats, were carried out. All four CHFs demonstrated genotoxic effects in both assays. In the two systems used, CMCF was the most genotoxic compound, followed by MCA, MX and MCF, respectively. This work was the first study of the DNA damaging properties of all four CHFs in two in vitro genotoxicity tests. These new data expand the range of genetic damages induced by this group of compounds.