Rodent model of nonarteritic ischemic optic neuropathy and its electrophysiological evaluation
Rodent model of nonarteritic ischemic optic neuropathy and its electrophysiological evaluation
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DOI：
10.1007/s10384-012-0167-y
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发表时间：
2012-09-01
影响因子：
2.4
通讯作者：
Nao-i, Nobuhisa
中科院分区：
文献类型：
--
作者：
Chuman, Hideki;Maekubo, Tomoyuki;Nao-i, Nobuhisa
我们的目的是建立非动脉炎性缺血性视神经病变（rNAION）的啮齿动物模型。为了诱导rNAION，在给予虎红（RB）（2.5 mM）后，使用光斑大小约为500 μ m的514 nm氩绿色激光光激活左侧视神经的小血管12 s（RB激光诱导）。为了评估诱导，眼底镜检查，荧光素血管造影（FA），可视化的视盘内的毛细血管，组织学评价，和电生理testings.In RB激光诱导的眼睛，视盘肿胀的第3天，然后萎缩的第8周。FA在早期显示脉络膜和视盘的充盈缺损，随后在晚期出现高荧光。视盘内毛细血管明显减少。组织学检查显示视盘前无细胞神经纤维层（NFL）肿胀。视网膜形态学变化仅在视网膜神经节细胞（RGC）层中明显，细胞数量减少。视觉诱发电位（VEP）波幅明显降低，而视网膜电图（ERG）无明显差异。阳性暗视阈值反应（pSTR）在诱导后第1天无明显降低，第3天明显降低，与人NAION相似。因此，RB-激光诱导非常适合于确定rNAION的存在。
Our aim was to establish a rodent model of nonarteritic ischemic optic neuropathy (rNAION).To induce rNAION, after administration of Rose Bengal (RB) (2.5 mM), the small vessels of the left optic nerve were photoactivated using a 514-nm argon green laser with about 500-mu m spot size for 12 s (RB-laser induction). To evaluate the induction, funduscopic examination, fluorescein angiography (FA), visualization of capillaries within the optic disc, histologic evaluation, and electrophysiological testing were performed.In the RB-laser-induction eyes, the optic disc became swollen on day 3 followed by atrophy on week 8. FA showed filling defects in the choroid and optic disc at an early stage, followed by hyperfluorescence at a late stage. The capillaries within the optic disc were reduced markedly. Histopathologic examination showed acellular nerve fiber layer (NFL) swelling anterior to the optic disc. The morphologic retinal changes were apparent only in the retinal ganglion cell (RGC) layer, with a reduction in the number of cells. Visually evoked potential (VEP) amplitude decreased significantly, but electroretinography (ERG) showed no significant difference. The positive scotopic threshold response (pSTR) was not reduced on the 1st day but was significantly reduced 3 days after induction.The findings are similar to human NAION. Therefore, RB-laser induction is well suited to establish the presence of rNAION.