Regulation of Gene Expression Using dCas9-SunTag Platforms
Regulation of Gene Expression Using dCas9-SunTag Platforms
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使用 dCas9-SunTag 平台调节基因表达
DOI：
10.1007/978-1-0716-2724-2_13
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Hatada I
中科院分区：
文献类型：
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作者：
Morita S;Horii T;Hatada I
调控基因表达对于基础研究和治疗应用是重要的。表观基因组是基因修饰的记录，如DNA甲基化和组蛋白修饰，表观遗传变化在修饰基因表达中起关键作用。随着基因组编辑技术的出现，操纵特定基因组区域的表观基因组以控制基因表达已经成为可能。特别地，CRISPR-Cas9系统由于其高效率、多功能性、特异性和易用性而广泛用于表观基因组编辑。在这里，我们描述了使用dCas 9-SunTag系统上调特定基因的方案。
Regulating gene expression is important for basic research and therapeutic applications. The epigenome is a record of genetic modifications such as DNA methylation and histone modifications, and epigenetic changes can play a key role in modifying gene expression. With the advent of genome editing technologies, it has become possible to manipulate the epigenome of specific genomic regions to control gene expression. In particular, CRISPR–Cas9 systems have been used widely for epigenome editing due to their high efficiency, versatility, specificity, and ease of use. Here, we describe a protocol for the upregulation of specific genes using the dCas9-SunTag system.