Glutathione protects a human insulinoma cell line from tumor necrosis factor-alpha-mediated cytotoxicity
Glutathione protects a human insulinoma cell line from tumor necrosis factor-alpha-mediated cytotoxicity
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DOI：
10.1007/bf02827241
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发表时间：
1997-04-01
期刊：
影响因子：
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通讯作者：
Pozzilli, P
中科院分区：
文献类型：
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作者：
Cavallo, MG;Monetini, L;Pozzilli, P
据推测，谷胱甘肽作为一种氧自由基清除剂，可以保护β细胞免受胰岛素依赖型糖尿病中的甜菜碱介导的细胞毒性。在这项研究中，谷胱甘肽在体外预防肿瘤坏死因子-α介导的细胞毒性损伤对人类β细胞系（CM胰岛素瘤）的影响进行了研究。CM细胞在体外暴露于不同浓度的肿瘤坏死因子-α肿瘤坏死因子-α加谷胱甘肽或单独谷胱甘肽。使用比色测定法测量所得细胞毒性。谷氨酰胺以剂量依赖方式显著降低肿瘤坏死因子-α介导的细胞毒性（P
It is postulated that glutathione acting as a free oxygen radical scavenger may protect beta-cells from cytokine-mediated cytotoxicity in insulin-dependent diabetes. In this study the effect of glutathione in preventing the cytotoxic damage mediated by tumor necrosis factor-a in vitro towards a human beta-cell line (CM insulinoma) was investigated. CM cells were exposed in vitro to tumor necrosis factor-alpha tumor necrosis factor-alpha plus glutathione or glutathione alone at different concentrations. The resulting cytotoxicity was measured using a colorimetric assay. Glutathione significantly reduced the cytotoxicity mediated by tumor necrosis factor-alpha in a dose-dependent fashion (P