Accumulation of gamma-aminobutyric acid in soybean by hypoxia germination and freeze-thawing incubation
Accumulation of gamma-aminobutyric acid in soybean by hypoxia germination and freeze-thawing incubation
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低氧发芽冻融培养大豆中γ-氨基丁酸的积累
DOI：
10.1002/jsfa.7323
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发表时间：
2016
影响因子：
4.1
通讯作者：
Gu Zhenxin
中科院分区：
文献类型：
--
作者：
Yang Runqiang;Feng Li;Wang Shufang;Yu Nanjing;Gu Zhenxin
γ -氨基丁酸（GABA）可以在低氧胁迫和低温条件下通过GABA分流和多胺降解途径合成。低温萌发冻融培养是提高大豆GABA积累量的新技术。结果在低氧条件下，种子萌发24 h内谷氨酸脱羧酶（GAD）、二胺氧化酶（DAO）活性和GABA含量均有所增加。干物质和蛋白质含量降低。经缺氧处理的芽在- 18°C冷冻12 h，在25°C解冻6 h， GABA含量急剧增加，达到未冷冻芽的7.21倍。随后，将冻融芽磨成匀浆并孵育。当匀浆在400µmol L - 1pyridoxine (VB6) （pH 6.5）中于45°C孵育80 min时，GABA的含量是仅浸泡过的种子的14.20倍。结论低温萌发冻融培养是积累大豆GABA的有效方法。在培养过程中，与GAD相比，DAO对萌发大豆匀浆中GABA的形成更为重要。©2015化学工业学会
BACKGROUNDγ‐Aminobutyric acid (GABA) can be synthesised by the GABA shunt and polyamine degradation pathway in plants under hypoxia stress and lower temperature. The hypoxia germination freeze–thawing incubation was used as a new technique for accumulating more GABA in soybean.RESULTSResults showed that glutamate decarboxylase (GAD) and diamine oxidase (DAO) activity as well as GABA content increased during germination within 24 h under hypoxia. However, the contents of dry matters and protein decreased. When the hypoxia‐treated sprouts were frozen at −18 °C for 12 h and thawed at 25 °C for 6 h, GABA content increased drastically to 7.21‐fold of the non‐frozen sprouts. Subsequently, the freeze–thawing sprouts were ground into homogenates and incubated. GABA content was 14.20‐fold of the only‐soaked seeds when homogenates was incubated at 45 °C for 80 min within 400 µmol L−1pyridoxine (VB6) (pH 6.5).CONCLUSIONThe hypoxia germination freeze–thawing incubation was an effective method for accumulating GABA in soybean. During incubation, DAO was more important for GABA formation in homogenate of germinated soybean compared with GAD. © 2015 Society of Chemical Industry