THE COMPLETE NUCLEOTIDE-SEQUENCE OF POTATO VIRUS-X AND ITS HOMOLOGIES AT THE AMINO-ACID LEVEL WITH VARIOUS PLUS-STRANDED RNA VIRUSES
THE COMPLETE NUCLEOTIDE-SEQUENCE OF POTATO VIRUS-X AND ITS HOMOLOGIES AT THE AMINO-ACID LEVEL WITH VARIOUS PLUS-STRANDED RNA VIRUSES
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DOI：
10.1099/0022-1317-69-8-1789
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发表时间：
1988-08-01
影响因子：
3.8
通讯作者：
CORNELISSEN, BJC
中科院分区：
文献类型：
--
作者：
HUISMAN, MJ;LINTHORST, HJM;CORNELISSEN, BJC
克隆了马铃薯X病毒(PVX)基因组RNA的双链cDNA，并进行了序列测定。序列[6435个核苷酸，不包括聚(A)链]显示了5个开放阅读框(ORF)，它们从RNA的5‘’末端开始编号为1到5。它们分别编码166K、24622(25K)、12324(12K)、7595(8K)和25080(外壳蛋白)的蛋白。ORF1和ORF2为同相编码区。ORF 1产物含有与烟草花叶病毒126K和183K产物同源的结构域。ORF 2和3分别与大麦条纹花叶病毒58K和14K蛋白、甜菜坏死性黄脉病毒42K和13K蛋白、白三叶花叶病毒26K和13K蛋白同源。这些同源性对于PVX编码蛋白的推测功能的意义进行了讨论。
Double-stranded cDNA of potato virus X (PVX) genomic RNA has been cloned and sequenced. The sequence [6435 nucleotides excluding the poly(A) tract] revealed five open reading frames (ORFs) which were numbered one to five starting at the 5'' terminus of the RNA. They encoded proteins of Mr 165 588 (166K), 24622 (25K), 12324 (12K), 7595 (8K) and 25080 (coat protein), respectively. ORFs 1 and 2 were inphase coding regions. The ORF 1 product contained domains of homology with the tobacco mosaic virus 126K and 183K products. The ORF 2 and 3 products showed homologies with the barley stripe mosaic virus 58K and 14K proteins, the beet necrotic yellow vein virus 42K and 13K products and the white clover mosaic virus 26K and 13K products, respectively. The significance of these homologies with respect to putative functions of the PVX-encoded proteins are discussed.