Superantigens and chronic rhinosinusitis II:: Analysis of T-cell receptor Vβ domains in nasal polyps
Superantigens and chronic rhinosinusitis II:: Analysis of T-cell receptor Vβ domains in nasal polyps
复制标题
DOI：
10.2500/ajr.2006.20.2880
复制
发表时间：
2006-07-01
期刊：
影响因子：
--
通讯作者：
Kern, Robert C.
中科院分区：
文献类型：
--
作者：
Conley, David B.;Tripathi, Anju;Kern, Robert C.
背景资料：慢性鼻窦炎（CRS）的超抗原（SAg）假说表明，鼻内毒素刺激T细胞群的大量寡克隆扩增，随后嗜酸性粒细胞募集和组织炎症。SAg能够通过结合T细胞受体（TCR）的特异性V β结构域来激活比常规抗原多1 × 10（4）的淋巴细胞。净效应是从正常的V β-配置文件的特定域的寡克隆扩增偏斜。本研究将评估息肉组织的证据，SAg反应在CRS鼻息肉（CRSwNP）。方法：本研究包括一个前瞻性分析18 CRSwNP受试者接受鼻窦手术。应用流式细胞术分析24个特异性TCR V β结构域在息肉组织和血液淋巴细胞中的分布。寡克隆扩增的证据制成表格，为每个标本，并定义为平均标准的百分比+2 SD.Results：18 CRSwNP受试者的息肉淋巴细胞表达TCR与特定的V β-域的扩张。每个CRSwNP受试者的V β-克隆的平均数是7个在息肉淋巴细胞，但只有两个在血液lymphocyte.Conclusion：目前的结果表明，息肉淋巴细胞表现出显着的寡克隆扩增特定的V β-域。这些数据被认为是SAg效应的诊断。相应的血液轮廓要少得多，这表明鼻子是刺激的主要来源。尽管这种扩增的触发因素尚不清楚，但这些数据与葡萄球菌毒素对CRSwNP的发展至关重要的假设一致。
Background: The superantigen (SAg) hypothesis of chronic rhinosinusitis (CRS) suggests that toxins within the nose stimulate massive oligoclonal expansion of T-cell populations with subsequent eosinophil recruitment and tissue inflammation. SAgs are capable of activating 1 x 10(4) more lymphocytes than conventional antigens by binding specific V beta-domains of the T-cell receptor (TCR). The net effect is skewing from the normal V beta-profile by oligoclonal expansion of specific domains. This study will assess polyp tissue for evidence of an SAg response in CRS with nasal polyps (CRSwNP).Methods: This study consists of a prospective analysis of 18 CRSwNP subjects undergoing sinus surgery. Flow cytometry was used to analyze the distribution of 24 specific TCR V beta-domains in lymphocytes from polyp tissue and blood. Evidence of oligoclonal expansion was tabulated for each specimen and defined as mean normative percentage + 2 SD.Results: Eighteen of 18 CRSwNP subjects showed expansion of polyp lymphocytes expressing TCRs with specific V beta-domains. The average number of V beta-clones per CRSwNP subject was seven in polyp lymphocytes but only two in blood lymphocytes.Conclusion: The current results indicate that polyp lymphocytes exhibit significant oligoclonal expansion of specific V beta-domains. These data are considered diagnostic of a SAg effect. The corresponding blood profile is much less, suggesting that the nose is the primary source of stimulus. Although the trigger(s)for this expansion are unknown, these data are consistent with the hypothesis that staphylococcal toxins are central to the development of CRSwNP.