Protein corona reduced graphene oxide cytotoxicity by inhibiting endocytosis
Protein corona reduced graphene oxide cytotoxicity by inhibiting endocytosis
复制标题
蛋白冠通过抑制内吞作用降低氧化石墨烯的细胞毒性
DOI：
10.1016/j.colcom.2021.100514
复制
发表时间：
2021-09-29
影响因子：
4.5
通讯作者：
Wen, Ling
中科院分区：
文献类型：
--
作者：
Yang, Ying;Han, Panli;Wen, Ling
随着石墨烯及其衍生物的广泛应用和生产，人们对石墨烯的接触越来越多。石墨烯可能暴露于各种生物微环境中，其中生物大分子的浓度变化。因此，研究石墨烯在不同生物微环境中的毒性行为是十分必要的。在本研究中，我们研究了氧化石墨烯（GO）在不同生物微环境中引起的毒性行为及其潜在机制。我们的研究结果表明，氧化石墨烯在无蛋白微环境中通过活性氧（ROS）诱导损伤细胞并诱导细胞凋亡。然而，在蛋白质的存在下，氧化石墨烯诱导的细胞毒性显著减弱，这归因于氧化石墨烯内化和ROS生成的减少。低浓度氧化石墨烯诱导细胞周期阻滞于G0/G1期，高浓度氧化石墨烯无明显细胞毒性。我们的研究结果为预防石墨烯毒性提供了基础。
Exposure to graphene is increasing with the extensive application and production of graphene and its derivatives. Graphene may be exposed to a variety of biomicroenvironments, in which the concentrations of bio-macromolecules vary. Thus, it is essential to study the toxic behaviors of graphene in different biomicroenvironments. In the present study, we investigated the toxic behaviors caused by graphene oxide (GO) in different biomicroenvironments and its potential mechanisms. Our results revealed that GO injured cells through reactive oxygen species (ROS) induction and induced apoptosis in protein-free microenvironment. However, GO-induced cytotoxicity was weakened remarkably in the presence of protein, which attributed to the reduction of GO internalization and ROS generation. GO induced cell cycle arrest in the G0/G1 phase when the protein concentration is low but had no remarkable cytotoxicity when the protein concentration was high. Our findings provide a basis for the prevention graphene toxicity.