Antibacterial Activity of Subtilosin Alone and Combined with Curcumin, Poly-Lysine and Zinc Lactate Against Listeria monocytogenes Strains
Antibacterial Activity of Subtilosin Alone and Combined with Curcumin, Poly-Lysine and Zinc Lactate Against Listeria monocytogenes Strains
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DOI：
10.1007/s12602-010-9042-7
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发表时间：
2010-12-01
影响因子：
4.9
通讯作者：
Chikindas, Michael L.
中科院分区：
文献类型：
--
作者：
Amrouche, Tahar;Noll, Katia Sutyak;Chikindas, Michael L.
本文研究了解淀粉芽孢杆菌产生的细菌素subtilosin单独使用以及与姜黄素、ε-聚L-赖氨酸或乳酸锌联合使用对单核细胞增生李斯特菌的抗菌作用。结果表明，枯草杆菌素抑制所研究的细菌菌株Scott A（野生型，乳链菌肽敏感性）和NR 30（乳链菌肽抗性）。然而，L.单核细胞增多症Scott A对枯草杆菌素和纯姜黄素更敏感。此外，subtilosin在酸性pH下更有活性。Subtilosin与包封的姜黄素组合显示出对L.单核细胞增多症ScottA.对两种L.单核细胞增多症Scott A和L.单核细胞增多症NR 30与乳酸锌的组合。当与未包封的姜黄素或聚赖氨酸组合时，仅观察到枯草杆菌素对所述菌株的累加效应。因此，使用subtilosin与姜黄素、聚赖氨酸或乳酸锌的组合，可以使用较低的有效剂量来控制L.单核细胞增多症我们的研究结果表明，subtilosin可以作为替代细菌素的乳链菌肽，提供了一个机会，使用一种新的天然和有效的生物防腐剂对L。单核细胞增多症在食品保存中的应用本文首次报道了枯草杆菌素与天然抗菌剂联合应用对L。单核细胞增多症
In this paper, the antibacterial effects of the Bacillus amyloliquefaciens-produced bacteriocin subtilosin, both alone and in combination with curcumin, epsilon-polyL-lysine (poly-lysine), or zinc lactate, were examined against Listeria monocytogenes. Results indicated that subtilosin inhibits both of the studied bacterial strains, Scott A (wild-type, nisin sensitive) and NR30 (nisin resistant). However, L. monocytogenes Scott A was more sensitive to subtilosin and pure curcumin. In addition, subtilosin was more active at an acidic pH. Subtilosin in combination with encapsulated curcumin displayed partial synergy against L. monocytogenes ScottA. It also had synergistic activity against both L. monocytogenes Scott A and L. monocytogenes NR30 when combined with zinc lactate. Only an additive effect was observed for subtilosin when combined with non-encapsulated curcumin or polylysine against the mentioned strains. Thus, using the combination of subtilosin with curcumin, poly-lysine, or zinc lactate, a lower effective dose can be used to control L. monocytogenes infection. Our findings suggest that subtilosin could be used as alternative bacteriocin to nisin, providing an opportunity to use a novel natural and efficacious biopreservative against L. monocytogenes in food preservation. This is the first report on the effects of the combination of subtilosin with natural antimicrobials on L. monocytogenes.