Saccharomyces cerevisiae as a model for the study of extranuclear functions of mammalian telomerase.
Saccharomyces cerevisiae as a model for the study of extranuclear functions of mammalian telomerase.
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DOI：
10.1007/s00294-014-0472-8
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发表时间：
2015-11
期刊：
影响因子：
2.5
通讯作者：
Tomaska, Lubomir
中科院分区：
文献类型：
--
作者：
Simonicova, Lucia;Dudekova, Henrieta;Ferenc, Jaroslav;Prochazkova, Katarina;Nebohacova, Martina;Dusinsky, Roman;Nosek, Jozef;Tomaska, Lubomir
The experimental evidence from the last decade made telomerase a prominent member of a family of moonlighting proteins performing different functions at various cellular loci. However, the study of extratelomeric function(s) of the catalytic subunit of mammalian telomerase (TERT) is often complicated by the fact that it is sometimes difficult to distinguish them from its role(s) at chromosomal ends. Here we describe an experimental model for studying extranuclear function(s) of mammalian telomerase in the yeast Saccharomyces cerevisiae. We demonstrate that the catalytic subunit of mammalian telomerase protects the yeast cells against oxidative stress and affect the stability of mitochondrial genome. The advantage of using S. cerevisiae for the study of mammalian telomerase is that (i) mammalian TERT does not interfere with its yeast counterpart in the maintenance of telomeres, (ii) yeast telomerase is not localized in mitochondria and (iii) it does not seem to be involved in the protection of the cells against oxidative stress and in the stabilization of mtDNA. Thus yeast cells can be used as a ‘test tube’ for reconstitution of mammalian TERT extranuclear function(s).
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1161/atvbaha.109.185546
发表时间：
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