Oxygen biosensor based on bilirubin oxidase immobilized on a nanostructured gold electrode
Oxygen biosensor based on bilirubin oxidase immobilized on a nanostructured gold electrode
复制标题
DOI：
10.1016/j.bioelechem.2013.07.001
复制
发表时间：
2013-12-01
影响因子：
5
通讯作者：
De Lacey, Antonio L.
中科院分区：
文献类型：
--
作者：
Pita, Marcos;Gutierrez-Sanchez, Cristina;De Lacey, Antonio L.
用金纳米颗粒修饰的金圆盘电极作为支架用于胆红素氧化酶的共价固定。纳米结构的生物电极作为无介质的生物传感器，用于模拟人体生理液体的含量和组成的缓冲液中的氧气。计时安培测量显示，对氧的检测限为6 +/- 1 μ M，线性范围为6-300 μ M，即超过人体组织和液体中氧的通常生理范围。该生物传感器是首次报道的基于胆红素氧化酶直接电子转移的氧安培生物传感器。(C) 2013 Elsevier B.V.版权所有
Gold disk electrodes modified with gold nanoparticles have been used as a scaffold for the covalent immobilization of bilirubin oxidase. The nanostructured bioelectrodes were tested as mediator-less biosensors for oxygen in a buffer that mimics the content and the composition of human physiological fluids. Chronoamperometry measurements showed a detection limit towards oxygen of 6 +/- 1 mu M with a linear range of 6-300 mu M, i.e. exceeding usual physiological ranges of oxygen in human tissues and fluids. The biosensor presented is the first ever-reported oxygen amperometric biosensor based on direct electron transfer of bilirubin oxidase. (C) 2013 Elsevier B.V. All rights reserved.