Initiation of germination of bacterial spores by hydrostatic pressure.
Initiation of germination of bacterial spores by hydrostatic pressure.
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通过静水压引发细菌孢子的萌发。
DOI：
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发表时间：
1970
期刊：
影响因子：
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通讯作者：
A. Sale
中科院分区：
文献类型：
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作者：
G. Gould;A. Sale
总结：静水压引发细菌孢子在无营养培养基中萌发。在1个大气压下对化学萌发剂休眠最强的孢子，对压力处理萌发的抗性也最强。通过高压处理的发芽的特征在于温度和pH最适，像在大气压下发芽。萌发开始的压力抑制代谢毒物和增强低浓度的各种营养物质，包括那些通常是发芽（在较高浓度）在大气压下。特别是，L-丙氨酸和密切相关的α-氨基酸，但不是他们的分解产物，加强发芽开始的压力。通过D-丙氨酸（其在I大气压下强烈抑制由L-丙氨酸引发的萌发）的增强作用的研究揭示，高压引起由孢子引起的丙氨酸外消旋化速率的增加。压力引起的萌发可能是由于某些萌发反应的加速，而这些反应在1个大气压的压力下通常是可以忽略不计的，也可能是由于孢子内某些屏障对L-丙氨酸和相关的α-氨基酸的渗透性增加。
SUMMARY: Hydrostatic pressure initiated germination of bacterial spores in nutrient-free media. Those spores which were most dormant towards chemical germinants at I atmosphere pressure were also the most resistant to germination by pressure treatment. Germination by high pressure treatment was characterized by temperature and pH optima, like germination at atmospheric pressure. Germination initiated by pressure was inhibited by metabolic poisons and was potentiated by low concentrations of various nutrients including some of those which are normally germinative (at higher concentrations) at atmospheric pressure. In particular, L-alanine and closely related α-amino acids, but not their breakdown products, potentiated germination initiated by pressure. Study of potentiation by D-alanine (which strongly inhibits germination initiated by L-alanine at I atmosphere pressure) revealed that high pressures caused an increase in the rate of racemization of alanine by spores. Germination by pressure probably resulted from acceleration of some germination reaction which is normally negligibly slow at a pressure of I atmosphere, and also from an increase in permeability of some barrier within the spore to L-alanine and related α-amino acids.