Construction of cDNA library from spleen of Scophthalmus maximus and identification of immune-related genes by EST sequencing.
Construction of cDNA library from spleen of Scophthalmus maximus and identification of immune-related genes by EST sequencing.
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发表时间：
2009
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通讯作者：
M. Liang;Chen Songlin;Liu Yang
中科院分区：
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作者：
M. Liang;Chen Songlin;Liu Yang
以大菱鲆（Scophthalmus maximus）脾脏为材料，采用单向克隆的方法构建了大菱鲆脾脏cDNA文库，对文库中3 656个EST进行了测序，并与GenBank中的序列进行了比较。（48.3%）似乎是完全未知的，可能代表新描述的基因，而1891个克隆这些被鉴定的克隆来自至少149个基因，这些基因被分为六类：9细胞结构/运动（6.0%），26例为代谢型（17.5%），细胞防御/免疫45基因/蛋白表达阳性43例（30.2%）（28.9%），16例细胞信号转导/通讯（10.7%），10个基因缺乏足够的信息进行分类（6.7%）。从脾中鉴定的免疫相关cDNA为：CC/CXC趋化因子、MHC I类α、补体成分C1/C3、自然杀伤细胞增强因子（NKEF）、胸腺素β-4/β-12、干扰素诱导蛋白Gig 1、IL-B4受体、STAT 4和热休克蛋白70/90。
A cDNA library was constructed from turbot,Scophthalmus maximus,spleen by unidirectional cloning.A total of 3 656 ESTs from the library were sequenced and compared with sequences in the GenBank database.1 765 clones(48.3%) appeared to be completely unknown and are likely to represent newly described genes,whereas 1 891 clones(51.7%) were identified based on matches to sequences in the database.These identified clones were derived from at least 149 genes which were categorized into six categories: 9 in cell structure/motility(6.0%),26 in metabolism(17.5%),45 in cell defense/immunity(30.2%),43 in gene/protein expression(28.9%),16 in cell signal transduction/communication(10.7%),and 10 genes lacking enough information to be classified(6.7%).Immune related cDNAs identified from the spleen were: CC/CXC chemokine,MHC class Iα,complement component C 1/C 3,natural killer cell enhancing factor(NKEF),thymosin beta-4/ beta-12,interferon inducible protein Gig1,IL-B4 receptor,STAT4,and hot shock protein 70/90.