Mutants of the Escherichia coli heat-labile enterotoxin with reduced ADP-ribosylation activity or no activity retain the immunogenic properties of the native holotoxin
Mutants of the Escherichia coli heat-labile enterotoxin with reduced ADP-ribosylation activity or no activity retain the immunogenic properties of the native holotoxin
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DOI：
10.1128/iai.64.12.5413-5416.1996
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发表时间：
1996-12-01
影响因子：
3.1
通讯作者：
Wilschut, J
中科院分区：
文献类型：
--
作者：
DeHaan, L;Verweij, WR;Wilschut, J
大肠杆菌不耐热肠毒素（LT）是一种强有力的黏膜免疫诱导剂。在以前的一项研究中（L。DeHaan，W. R. Verweij，M. Holtrop，E. Agsteribbe和J. Wilschut，Vaccine 14：620-626，1996），我们已经表明LT对LTB特异性粘膜免疫应答的有效诱导需要LTA链的存在，这表明LTA的酶活性在这些应答的诱导中可能起作用。在本研究中，我们产生了LT突变体，vith改变ADP-核糖基化活性，并评估其免疫原性后，鼻内给药小鼠。结果表明，LT的粘膜免疫原性不依赖于其ADP-核糖基化活性。
The Escherichia coli heat-labile enterotoxin (LT) is a potent inducer of mucosal immune responses. In a previous study (L. DeHaan, W. R. Verweij, M. Holtrop, E. Agsteribbe, and J. Wilschut, Vaccine 14:620-626, 1996), we have shown that efficient induction of an LTB-specific mucosal immune response by LT requires the presence of the LTA chain, suggesting a possible role of the enzymatic activity of LTA in the induction of these responses. In the present study, we generated LT mutants,vith altered ADP-ribosylation activities and evaluated their immunogenicity upon intranasal administration to mice. The results demonstrate that the mucosal immunogenicity of LT is not dependent on its ADP-ribosylation activity.