Thermo-responsive polymer brush-grafted porous polystyrene beads for all-aqueous chromatography
Thermo-responsive polymer brush-grafted porous polystyrene beads for all-aqueous chromatography
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DOI：
10.1016/j.chroma.2009.11.073
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发表时间：
2010-01-22
影响因子：
4.1
通讯作者：
Okano, Teruo
中科院分区：
文献类型：
--
作者：
Mizutani, Aya;Nagase, Kenichi;Okano, Teruo
采用表面引发原子转移自由基聚合（ATRP）制备了具有不同接枝聚合物密度的聚（N-异丙基丙烯酰胺）（PIPAAm）刷状接枝多孔聚苯乙烯珠，用于温敏色谱。在胰岛素的水性色谱分析中利用这些接枝珠作为固定相提供了接枝密度依赖性分析物保留行为。PIPAAm接枝的聚苯乙烯上的分离校准曲线使用支链淀粉标准品获得，并且表现出归因于分析物扩散到珠孔中并分配到接枝的PIPAAm刷表面中的拐点。这些拐点的存在支持分离机制，其中胰岛素渗透聚苯乙烯珠中的孔并与孔内接枝的PIPAAm刷疏水相互作用。聚苯乙烯上PIPAAm刷接枝密度的控制促进了基于与固定相孔内接枝的PIPAAm的热调节疏水相互作用的肽的有效水相分离。这些结果表明，通过表面引发ATRP制备的PIPAAm刷接枝多孔聚苯乙烯珠是有效的水相肽分离的热响应色谱固定相。(C)2009爱思唯尔有限公司版权所有。
Poly(N-isopropylacrylamide) (PIPAAm) brush-grafted porous polystyrene beads with variable grafted polymer densities were prepared using surface-initiated atom transfer radical polymerization (ATRP) for applications in thermo-responsive chromatography. Utilization of these grafted beads as a stationary phase in aqueous chromatographic analysis of insulin provides a graft density-dependent analyte retention behavior. The separations calibration curve on PIPAAm-grafted polystyrene was obtained using pullulan standards and exhibited inflection points attributed to analyte diffusion into bead pores and partitioning into grafted PIPAAm brush surfaces. Presence of these inflection points supports a separation mechanism where insulin penetrates pores in polystyrene beads and hydrophobically interacts with PIPAAm brushes grafted within the pores. Control of PIPAAm brush graft density on polystyrene facilitates effective aqueous phase separation of peptides based on thermally modulated hydrophobic interactions with grafted PIPAAm within stationary phase pores. These results indicated that PIPAAm brush-grafted porous polystyrene beads prepared by surface-initiated ATRP was effective stationary phase of thermo-responsive chromatography for aqueous phase peptide separations. (C) 2009 Elsevier B.V. All rights reserved.