Polyploidy and microbiome associations mediate similar responses to pathogens in Arabidopsis
Polyploidy and microbiome associations mediate similar responses to pathogens in Arabidopsis
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DOI：
10.1016/j.cub.2022.05.015
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发表时间：
2022-05
期刊：
影响因子：
9.2
通讯作者：
Elijah C. Mehlferber;Michael J. Song;Julianne N. Pelaez;J. Jaenisch;J. Coate;B. Koskella;C. Rothfels
中科院分区：
文献类型：
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作者：
Elijah C. Mehlferber;Michael J. Song;Julianne N. Pelaez;J. Jaenisch;J. Coate;B. Koskella;C. Rothfels
越来越清楚的是，微生物组在塑造宿主生物体对疾病的反应方面起着关键作用。还有越来越多的证据表明，生物体的倍性水平在它们对病原体和寄生虫的反应中是重要的。然而，没有研究确定这两个因素是否或如何相互影响。我们研究了拟南芥全基因组复制对地上（叶圈）微生物组的影响，并确定了倍性和微生物组对疾病结果的相互影响。使用7个独立衍生的合成autotetraploidalphanopsis加入和合成的叶相关的细菌群落，我们证实，多倍体通常更耐模型病原体假单胞菌pepv。番茄DC3000无论微生物接种如何，多倍体都比二倍体更好地对抗病原体，而具有完整微生物组的二倍体比没有微生物组的二倍体具有更低的病原体密度。此外，二倍体具有增加的防御相关基因的数量，这些基因在其叶际微生物群的存在下差异表达，而多倍体则表现出一些组成性激活的防御，而不管合成群落的定殖。这些结果表明，全基因组复制可以增强免疫力，从而减少对微生物组的依赖以保护免受病原体的侵害。
It has become increasingly clear that the microbiome plays a critical role in shaping the host organism's response to disease. There also exists mounting evidence that an organism's ploidy level is important in their response to pathogens and parasites. However, no study has determined whether or how these two factors influence one another. We investigate the effect of whole-genome duplication inArabidopsis thalianaon the above-ground (phyllosphere) microbiome and determine the interacting impacts of ploidy and microbiome on disease outcome. Using seven independently derived synthetic autotetraploidArabidopsisaccessions and a synthetic leaf-associated bacterial community, we confirm that polyploids are generally more resistant to the model pathogenPseudomonas syringaepv. Tomato DC3000. Polyploids fare better against the pathogen than diploids do, regardless of microbial inoculation, whereas diploids harboring an intact microbiome have lower pathogen densities than those without. In addition, diploids have elevated numbers of defense-related genes that are differentially expressed in the presence of their phyllosphere microbiota, whereas polyploids exhibit some constitutively activated defenses, regardless of colonization by the synthetic community. These results imply that whole-genome duplication can enhance immunity, resulting in a decreased dependence on the microbiome for protection against pathogens.