Growth and morphologic response of rumen methanogenic archaea and bacteria to cashew nut shell liquid and its alkylphenol components.
Growth and morphologic response of rumen methanogenic archaea and bacteria to cashew nut shell liquid and its alkylphenol components.
复制标题
瘤胃产甲烷古菌和细菌对腰果壳液及其烷基酚成分的生长和形态响应。
DOI：
10.1111/asj.13598
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发表时间：
2021
影响因子：
2
通讯作者：
Y.
中科院分区：
文献类型：
--
作者：
Wakai;M.;Hayashi;S.;Chiba;Y.;Koike;S.;Nagashima;K.;Kobayashi;Y.
采用体外纯培养和添加腰果壳液（CNSL）及其酚类化合物（反刍动物用抗甲烷生成剂）的培养物，研究了瘤胃产甲烷古菌（5属10种15株，其中包括7株新分离菌株）和细菌（12属14种）的生长和形态。在1.56 ~ 12.5 μg/ml浓度范围内，CNSL和烷基酚对大多数产甲烷菌的生长均有抑制作用。在测试的烷基酚中，漆树酸表现出最有效的生长抑制作用。参与丙酸盐生产的三种革兰氏阴性细菌对CNSL和烷基酚（>50 μg/ml）具有抗性。所有对CNSL和烷基酚敏感的产甲烷菌和细菌均表现出形态改变;细胞表面的破坏是显著的，可能是由于测试材料的表面活性剂活性。在某些生物体中，细胞分裂受到抑制，观察到细胞伸长和不清楚的隔膜形成。这些结果表明，CNSL和烷基酚，特别是漆树酸，以选择性的方式抑制瘤胃细菌和产甲烷菌，这可能有助于减少瘤胃甲烷的产生。
The growth and morphology of rumen methanogenic archaea (15 strains of 10 species in 5 genera, including 7 strains newly isolated in the present study) and bacteria (14 species in 12 genera) were investigated using unsupplemented in vitro pure cultures and cultures supplemented with cashew nut shell liquid (CNSL) and its phenolic compound components, anti‐methanogenic agents for ruminant animals. Growth of most of the methanogens tested was inhibited by CNSL and alkylphenols at different concentrations ranging from 1.56 to 12.5 μg/ml. Of the alkylphenols tested, anacardic acid exhibited the most potent growth inhibition. Three gram‐negative bacterial species involved in propionate production were resistant to CNSL and alkylphenols (>50 μg/ml). All the methanogens and bacteria that were sensitive to CNSL and alkylphenols exhibited altered morphology; disruption of the cell surface was notable, possibly due to surfactant activity of the tested materials. Cells division was inhibited in some organisms, with cell elongation and unclear septum formation observed. These results indicate that CNSL and alkylphenols, particularly anacardic acid, inhibit both rumen bacteria and methanogens in a selective manner, which could help mitigate rumen methane generation.