Uncoupling protein 2 protects testicular germ cells from hyperthermia-induced apoptosis.
Uncoupling protein 2 protects testicular germ cells from hyperthermia-induced apoptosis.
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DOI：
10.1016/j.bbrc.2007.06.071
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发表时间：
2007-08
影响因子：
3.1
通讯作者：
Keying Zhang;Y. Shang;Shangying Liao;Wei Zhang;Hongqiang Nian;Yi-Xun Liu;Quan Chen;Chunsheng Han
中科院分区：
文献类型：
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作者：
Keying Zhang;Y. Shang;Shangying Liao;Wei Zhang;Hongqiang Nian;Yi-Xun Liu;Quan Chen;Chunsheng Han
线粒体解偶联蛋白2（UCP 2）的mRNA上调隐睾，睾丸过热条件下，生殖细胞发生严重的凋亡。我们研究了UCP 2是否能够保护生殖细胞免受高血压诱导的凋亡。UCP 2在正常条件下主要存在于精子细胞中，在所有生殖细胞中均可检测到，如果睾丸暴露于43°C 5 min，其水平显著升高。如此短的热暴露是非致命的，并且使预处理的细胞能够抵抗较长时间的高温处理（15分钟）诱导的细胞凋亡。虽然高温导致小鼠睾丸的氧化应激，但它并没有改变总的抗氧化能力。事实上，在GC-2生殖细胞系中UCP 2的过表达保护细胞免受自由基氧（ROS）诱导的凋亡。综上所述，我们认为UCP 2可能是生殖细胞对抗ROS诱导的细胞凋亡的有效武器。
The mRNA of the mitochondrial uncoupling protein 2 (UCP2) was up-regulated by cryptorchidism, a testicular hyperthermic condition under which germ cells undergo severe apoptosis. We investigated whether UCP2 was able to protect germ cells from hyperthermia-induced apoptosis. UCP2 was predominantly present in elongate spermatids under normal conditions, and was detected in all germ cells with its level significantly increased if the testes were exposed to 43°C for 5min. Such a short heat exposure was non-lethal and enabled the preconditioned cells to be resistant to apoptosis induced by a longer hyperthermic treatment (15min). While hyperthermia resulted in oxidative stress in mouse testes, it did not change the total anti-oxidative capacity. Indeed, overexpression of UCP2 in the GC-2 germ cell line protected the cells from radical oxygen species (ROS)-induced apoptosis. Taken together, we propose that UCP2 may represent an effective weaponry used by germ cells to combat ROS-induced apoptosis.