The vacuolar H+/Ca transporter CAX1 participates in submergence and anoxia stress responses.
The vacuolar H+/Ca transporter CAX1 participates in submergence and anoxia stress responses.
复制标题
液泡 H /Ca 转运蛋白 CAX1 参与淹没和缺氧应激反应。
DOI：
10.1093/plphys/kiac375
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发表时间：
2022
期刊：
影响因子：
7.4
通讯作者：
Hirschi,KendalD
中科院分区：
文献类型：
--
作者：
Yang,Jian;Mathew,InyElizebeth;Rhein,Hormat;Barker,Richard;Guo,Qi;Brunello,Luca;Loreti,Elena;Barkla,BronwynJ;Gilroy,Simon;Perata,Pierdomenico;Hirschi,KendalD
植物的氧气供应可以从正常（常氧）到完全耗尽（缺氧）。耐缺氧性与湿地物种、水稻栽培和作物耐淹性有关。钙信号的解码和传递可能是缺氧耐受的重要组成部分，然而，细胞内钙转运蛋白对这一过程的贡献知之甚少。拟南芥（Arabidopsis thaliana）中的四种功能性阳离子/质子交换体（CAX 1 -4）有助于调节液泡周围的Ca稳态。我们的研究结果表明，cax 1突变体更耐缺氧条件和淹没。使用表型测量，RNA测序和蛋白质组学方法，我们确定了cax 1介导的缺氧变化，耐缺氧作物中存在的表型变化：代谢过程改变，缺氧后活性氧产生减少，激素信号改变。比较野生型和cax 1表达基因编码的钙指示剂表明，在复氧过程中，cax 1胞浆钙信号发生改变。缺氧诱导的植物液泡周围的钙信号参与了许多与低氧胁迫适应相关的信号事件的控制。这项工作表明，cax 1缺氧反应途径可以被工程化，以规避洪水的不利影响，损害生产农业。
A plant’s oxygen supply can vary from normal (normoxia) to total depletion (anoxia). Tolerance to anoxia is relevant to wetland species, rice (Oryza sativa) cultivation, and submergence tolerance of crops. Decoding and transmitting calcium (Ca) signals may be an important component to anoxia tolerance; however, the contribution of intracellular Ca transporters to this process is poorly understood. Four functionalcation/proton exchangers (CAX1–4) in Arabidopsis (Arabidopsis thaliana) help regulate Ca homeostasis around the vacuole. Our results demonstrate thatcax1mutants are more tolerant to both anoxic conditions and submergence. Using phenotypic measurements, RNA-sequencing, and proteomic approaches, we identifiedcax1-mediated anoxia changes that phenocopy changes present in anoxia-tolerant crops: altered metabolic processes, diminished reactive oxygen species production post anoxia, and altered hormone signaling. Comparing wild-type andcax1expressing genetically encoded Ca indicators demonstrated altered cytosolic Ca signals incax1during reoxygenation. Anoxia-induced Ca signals around the plant vacuole are involved in the control of numerous signaling events related to adaptation to low oxygen stress. This work suggests thatcax1anoxia response pathway could be engineered to circumvent the adverse effects of flooding that impair production agriculture.