Interaction of integrins alpha 3 beta 1 and alpha 2 beta 1: potential role in keratinocyte intercellular adhesion.
Interaction of integrins alpha 3 beta 1 and alpha 2 beta 1: potential role in keratinocyte intercellular adhesion.
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整合素 α 3 β 1 和 α 2 β 1 的相互作用：在角质形成细胞细胞间粘附中的潜在作用。
DOI：
10.1083/jcb.120.2.523
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Carter,WG
中科院分区：
文献类型：
--
作者：
Symington,BE;Takada,Y;Carter,WG
整合素α2β1和α3β1在角质形成细胞的细胞间接触部位和表皮中的共存提示，这些整合素可能介导细胞间黏附。P1B5是一种抗α3β1单抗，已有报道可抑制角质形成细胞与外胚层的黏附，也被发现可诱导ICA。通过ICA分析和纯化的mAb固定的整合素的分析，获得了P1B5诱导的ICA是由α2β1和α3β1介导的证据。观察到包被α2β1的微珠选择性地与表皮细胞或平板结合的α3β1结合。这种结合被单抗抑制整合素α3、α2或β1亚基，并可被P1B5刺激。我们还证明了亲和纯化的整合素α2β1和α3β1之间的选择性和可抑制的相互作用。最后，我们证明了CHO成纤维细胞表达α2β1导致胶原的获得和α3β1的结合。与这两种配体的结合被P1H5抑制，P1H5是一种抗α2β1特异性mAb。这些体外实验的结果表明，整合素α2β1和α3β1可以相互作用，并可能在体内介导ICA。因此，α3β1介导角质形成细胞与外胚层的黏附，并通过α2β1在ICA中发挥第二作用。
The colocalization of integrins alpha 2 beta 1 and alpha 3 beta 1 at intercellular contact sites of keratinocytes in culture and in epidermis suggests that these integrins may mediate intercellular adhesion (ICA). P1B5, an anti-alpha 3 beta 1 mAb previously reported to inhibit keratinocyte adhesion to epiligrin, was also found to induce ICA. Evidence that P1B5-induced ICA was mediated by alpha 2 beta 1 and alpha 3 beta 1 was obtained using both ICA assays and assays with purified, mAb-immobilized integrins. Selective binding of alpha 2 beta 1-coated beads to epidermal cells or plate-bound alpha 3 beta 1 was observed. This binding was inhibited by mAbs to integrin alpha 3, alpha 2, or beta 1 subunits and could be stimulated by P1B5. We also demonstrate a selective and inhibitable interaction between affinity-purified integrins alpha 2 beta 1 and alpha 3 beta 1. Finally, we show that expression of alpha 2 beta 1 by CHO fibroblasts results in the acquisition of collagen and alpha 3 beta 1 binding. Binding to both of these ligands is inhibited by P1H5, an anti-alpha 2 beta 1 specific mAb. Results of these in vitro experiments suggest that integrins alpha 2 beta 1 and alpha 3 beta 1 can interact and may do so to mediate ICA in vivo. Thus, alpha 3 beta 1 mediates keratinocyte adhesion to epiligrin and plays a second role in ICA via alpha 2 beta 1.