Simultaneous qualitative and quantitative analysis of phenolic acids and flavonoids for the quality control of Apocynum venetum L. leaves by HPLC-DAD-ESI-IT-TOF-MS and HPLC-DAD
Simultaneous qualitative and quantitative analysis of phenolic acids and flavonoids for the quality control of Apocynum venetum L. leaves by HPLC-DAD-ESI-IT-TOF-MS and HPLC-DAD
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DOI：
10.1016/j.jpba.2013.07.005
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发表时间：
2013-11-01
影响因子：
3.4
通讯作者：
Chen, Shizhong
中科院分区：
文献类型：
--
作者：
An, Haijuan;Wang, Hong;Chen, Shizhong
建立了高效液相色谱-二极管阵列检测器-电喷雾电离-离子阱-飞行时间-质谱（HPLC-DAD-ESI-IT-TOF-MS）分析罗布麻中酚酸类和黄酮类化合物的方法。叶及其混伪品，大花罗布麻Pocynumhendersonii（Hook.）f.）伍德森离开了。共鉴定或初步鉴定出21种化合物，其中酚酸类4种，黄酮类17种。3-O-咖啡酰奎尼酸（3-CQA）和咖啡酸是首次在A.威尼斯叶;首次从山茱萸叶中检出4-O-咖啡酰奎宁酸（4-CQA）、3-CQA、咖啡酸、槲皮素-3-O-（6“-O-丙二酰）-半乳糖苷、槲皮素-3-O-（6 "-O-丙二酰）-葡萄糖苷、山萘酚-3-O-（6”-O-丙二酰）-葡萄糖苷、山萘酚-3-O-（6“-O-乙酰基）-葡萄糖苷和山萘酚-3-O-二己糖苷。对24批A.从中国不同地区采集的5批三叶草叶和5批三叶草叶。基于21种化合物的分析表明，这些化合物的谱在两个物种之间以及A.来自不同产地的马鞭草叶样本。从这21个化合物中筛选出18个化合物作为标记物，首次采用HPLC-DAD法进行了同时分析。对所建立的定量分析方法进行了验证，并应用于24批A.委内瑞拉的叶子。(C)2013爱思唯尔有限公司版权所有。
A reliable method based on high performance liquid chromatography-diode array detector-electrospray ionization-ion trap-time of flight-mass spectrometry (HPLC-DAD-ESI-IT-TOF-MS) was developed for the identification of phenolic acids and flavonoids in Apocynum venetum L. leaves and its adulterant, Pocynum hendersonii (Hook. f.) Woodson leaves. A total of 21 compounds were identified or tentatively identified, including 4 phenolic acids and 17 flavonoids. 3-O-caffeoylquinic acid (3-CQA) and caffeic acid were detected for the first time in A. venetum leaves; 4-O-caffeoylquinic acid (4-CQA), 3-CQA, caffeic acid, quercetin-3-O-(6 ''-O-malonyl)-galactoside, quercetin-3-O-(6 ''-O-malonyl)-glucoside, kaempferol-3-O-(6 ''-O-malonyl)-glucoside, kaempferol-3-O-(6 ''-O-acetyl)-glucoside, and kaempferol-3-O-dihexoside were detected for the first time in P. hendersonii leaves. Cluster analysis was employed to analyze 24 batches of A. venetum leaves and 5 batches of P. hendersonii leaves collected from various regions in China. The analysis, which was based on the 21 compounds, indicated that profiles of these compounds were distinct between the two species, and among A. venetum leaf samples from different origins. 18 of these 21 compounds were selected as the markers and simultaneously analyzed by HPLC-DAD for the first time. The quantitative analytical method was validated and subsequently applied to the comprehensive quality evaluation of 24 batches of A. venetum leaves. (C) 2013 Elsevier B.V. All rights reserved.