A NEW ASSAY FOR LIGNIN-TYPE PEROXIDASES EMPLOYING THE DYE AZURE-B
A NEW ASSAY FOR LIGNIN-TYPE PEROXIDASES EMPLOYING THE DYE AZURE-B
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DOI：
10.1128/aem.58.9.3110-3116.1992
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发表时间：
1992-09-01
影响因子：
4.4
通讯作者：
ARCHIBALD, FS
中科院分区：
文献类型：
--
作者：
ARCHIBALD, FS
1983年发现的真菌“木质素酶”能够催化非酚芳香族木质素组分的过氧化，这被视为理解某些担子菌真菌如何完全降解木质素的一个重大进展。这些木质素型过氧化物酶将毫摩尔浓度的藜芦醇转化为藜芦醛的能力，由藜芦醛的A310的变化表示，已经成为LP活性的常规定量的标准测定。提出了一种基于微摩尔浓度的染料天青B的氧化的新测定法。虽然它与藜芦醇测定法一样简单快速，但它似乎克服了该测定法的一些缺点。特别是，来自紫外线和短波长可见光吸收材料的干扰大大减少，并提高了测定特异性。
The discovery in 1983 of fungal "ligninases" capable of catalyzing the peroxidation of nonphenolic aromatic lignin components has been seen as a major advance in understanding how certain basidiomycete fungi can completely degrade lignin. The ability of these lignin-type peroxidases to convert millimolar concentrations of veratryl alcohol to veratraldehyde, indicated by a change in the A310 of veratraldehyde, has become the standard assay for routine quantitation of LP activity. A new assay based on the oxidation of micromolar concentrations of the dye Azure B is presented. Although it is as simple and rapid as the veratryl alcohol assay, it appears to overcome some of the shortcomings of that assay. In particular, interference from UV- and short-wavelength visible-light-absorbing materials is greatly reduced and assay specificity is improved.