Characterization of Long Non-coding RNA Associated Proteins by RNA-Immunoprecipitation.
Characterization of Long Non-coding RNA Associated Proteins by RNA-Immunoprecipitation.
复制标题
通过 RNA 免疫沉淀表征长链非编码 RNA 相关蛋白。
DOI：
10.1007/978-1-0716-1697-0_3
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Zhang,Youyou
中科院分区：
文献类型：
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作者：
Jiang,Junjie;Zhang,Tianli;Pan,Yutian;Hu,Zhongyi;Yuan,Jiao;Hu,Xiaowen;Zhang,Lin;Zhang,Youyou
With the advances in sequencing technology and transcriptome analysis, it is estimated that up to 75% of the human genome is transcribed into RNAs. This finding prompted intensive investigations on the biological functions of non-coding RNAs and led to very exciting discoveries of microRNAs as important players in disease pathogenesis and therapeutic applications. Research on long non-coding RNAs (lncRNAs) is in its infancy, yet a broad spectrum of biological regulations has been attributed to lncRNAs. RNA-immunoprecipitation (RNA-IP) is a technique of detecting the association of individual proteins with specific RNA molecules in vivo. It can be used to investigate lncRNA–protein interaction and identify lncRNAs that bind to a protein of interest. Here we describe the protocol of this assay with detailed materials and methods.
