An Enzyme Linked Immunosorbent Assay for Determination of Tissue Plasminogen Activator Applied to Patients with Thromboembolic Disease
An Enzyme Linked Immunosorbent Assay for Determination of Tissue Plasminogen Activator Applied to Patients with Thromboembolic Disease
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用于测定血栓栓塞性疾病患者组织纤溶酶原激活剂的酶联免疫吸附测定
作者：
N. Bergsdorf;T. Nilsson;P. Wallén
利用抗人子宫组织纤溶酶原激活剂的山羊抗血清中的免疫球蛋白组份，建立了检测人血浆中组织型纤溶酶原激活剂的酶联免疫分析方法。用此法测得正常人酸化血浆中t-PA浓度为4.0±1.8(SD)ng/ml，试管试验后为12 ng/ml，剧烈运动后为14 ng/ml。在一组特发性血栓栓塞症患者中，静息t-PA浓度为5 ng/ml，闭塞后t-PA浓度为16 ng/ml，且闭塞后纤溶酶-α-2-抗纤溶酶复合体浓度正常升高。然而，在37%的闭塞后患者血浆中，通过特定的活性检测几乎没有检测到t-PA的增加。结果表明，在大多数情况下，闭塞后纤溶活性缺陷的原因可能是快速作用的特异性t-PA抑制物浓度增加。
Summary Utilizing the immunoglobulin fraction from a goat antiserum against human uterine tissue plasminogen activator, an enzyme- linked immunoassay for tissue-type plasminogen activator in human plasma has been developed. With the new method, the concentration of t-PA in normal human acidified plasma is found to be 4.0 ± 1.8 (SD) ng/ml. It increases to 12 ng/ml after a tomiquet test, and to 14 ng/ml after strenous physical exercise. In a group of patients with idiopathic thromboembolic disease, the resting t-PA concentration was 5 ng/ml and the post-occlusion value 16 ng/ml. Furthermore, the patients also exhibited a normal post-occlusion rise in the concentration of plasmin-α2-antiplasmin complex. However, in 37% of the post-occlusion patient plasmas, virtually no increase in t-PA could be detected by a specific activity assay. The results indicate that the reason for a defective post-occlusion fibrinolytic activity in a majority of cases may be the presence of increased concentrations of a fast-acting specific t-PA inhibitor.