Strong genetic admixture in the Altai at the Middle Bronze Age revealed by uniparental and ancestry informative markers
Strong genetic admixture in the Altai at the Middle Bronze Age revealed by uniparental and ancestry informative markers
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DOI：
10.1016/j.fsigen.2014.05.012
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发表时间：
2014-09-01
影响因子：
3.1
通讯作者：
Ludes, Bertrand
中科院分区：
文献类型：
--
作者：
Hollard, Clemence;Keyser, Christine;Ludes, Bertrand
阿尔泰山脉是欧亚草原种群与南亚和东亚种群之间的长期边界地带。为了解开该地区历史上的人口流动，研究了在蒙古阿尔泰最西部出土的14具古代人类标本。其中13件的年代为青铜时代中期至末期，1件的年代为新石器时代。该地区的环境条件使人类遗骸中的DNA保存良好。因此，采用多标记的方法进行身份、祖先和表型标记的遗传分析。线粒体DNA分析显示，研究的古代阿尔泰人携带西方（H，U，T）和东方（A，C，D）欧亚血统。以同样的方式，父系基因库揭示了不同单倍型群（Q1 a2 a1-L54，R1 a1 a1 b2-Z93和C）的存在，可能标志着男性父系谱系的不同起源。为了进一步研究这些古代标本的起源，确定了表型特征（即头发和眼睛的颜色）。为此目的，我们适应了最近描述的HIRI测定MALDI-TOF质谱法。此外，一些祖先的信息标记进行了分析与此测定。结果显示，这组之间的混合表型确认可能的混合祖先的研究阿尔泰人口在青铜时代中期。从古代DNA样本中获得的良好结果表明，这种方法也可能与法医案例有关。(C)2014爱思唯尔爱尔兰有限公司版权所有。
The Altai Mountains have been a long-term boundary zone between the Eurasian Steppe populations and South and East Asian populations. To disentangle some of the historical population movements in this area, 14 ancient human specimens excavated in the westernmost part of the Mongolian Altai were studied. Thirteen of them were dated from the Middle to the End of the Bronze Age and one of them to the Eneolithic period. The environmental conditions encountered in this region led to the good preservation of DNA in the human remains. Therefore, a multi-markers approach was adopted for the genetic analysis of identity, ancestry and phenotype markers. Mitochondrial DNA analyses revealed that the ancient Altaians studied carried both Western (H, U, T) and Eastern (A, C, D) Eurasian lineages. In the same way, the patrilineal gene pool revealed the presence of different haplogroups (Q1a2a1-L54, R1a1a1b2-Z93 and C), probably marking different origins for the male paternal lineages. To go further in the search of the origin of these ancient specimens, phenotypical characters (i.e. hair and eye color) were determined. For this purpose, we adapted the HIrisPlex assay recently described to MALDI-TOF mass spectrometry. In addition, some ancestry informative markers were analyzed with this assay. The results revealed mixed phenotypes among this group confirming the probable admixed ancestry of the studied Altaian population at the Middle Bronze Age. The good results obtained from ancient DNA samples suggest that this approach might be relevant for forensic casework too. (C) 2014 Elsevier Ireland Ltd. All rights reserved.