Low incidence of Epstein–Barr virus presence in primary cutaneous T‐cell lymphoproliferations
Low incidence of Epstein–Barr virus presence in primary cutaneous T‐cell lymphoproliferations
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原发性皮肤 T-细胞淋巴细胞增殖中 Epstein-Barr 病毒的发生率较低
DOI：
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发表时间：
1996
影响因子：
10.3
通讯作者：
Harald Stein
中科院分区：
文献类型：
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作者：
I. Anagnostopoulos;Michael Hummel;P. Kaudewitz;P. Korbjuhn;Lorenzo Leoncini;Harald Stein
总结从76例欧洲人类免疫缺陷病毒（HIV）阴性原发性皮肤T细胞淋巴增生患者中采集的多例活检，包括蕈样肉芽肿（MF）、多形性T细胞淋巴瘤（PMTCL）。通过联合方法研究了间变性大细胞腺瘤（ALCLI）和淋巴瘤样丘疹病（LyP）中Epstein巴尔病毒（EBV）的存在。采用聚合酶链反应（PCR）检测EBV-DNA，原位杂交（ISH）检测EBV编码的核RNA（EBKR 1和EBER 2）和立即早期Bam H-片段：下框（BHLF）RNA的细胞定位。和免疫组织学（IH）用于鉴定EBV编码的潜伏膜蛋白1（LMP 1）和核抗原（EBNA）2表达。在76例病例中，15例（19.7%）通过PCR检测到EBV-DNA。EBER-ISH结合IH在15例EBV-ONA-携带病例中仅7例中发现了可变的（通常非常低的）感染肿瘤细胞数量。用PCR和ISH获得的结果之间的这种差异显然是由每个组织切片的感染细胞数量少引起的。PMTCL实体产生了最多的阳性病例，而ALCL和LyP病例几乎总是没有病毒。在任何情况下都检测不到BHLF转录物，也没有任何EBER阳性细胞显示LMP 1或EBNA 2表达。这些数据表明，原发性皮肤T淋巴细胞增生与潜伏性EBV感染的相关性不常见，并且病毒在阳性病例中的致病作用仍然不清楚，因为病毒经常只感染少数非典型细胞。
Summary Multíple bíopsíes taken from 76 European human Immunodeficiency virus (HIV)‐negative patients with primary cutaneous T‐cell lymphoproliferations, including mycosis fungoides (MF), pleomorphic T‐cell lymphoma (PMTCL). anaplastic large cell lympboma (ALCLI and lymphomatoid papulosis (LyP) were investigated for the presence of Epstein‐Barr virus (EBV) through a combined approach. Polymerase chain reaction (PCR) was employed for EBV‐DNA detection, in situ hybridization (ISH) for cellular localization of EBV‐encoded nuclear RNAs (EBKR1 and EBER2) and immediate early Bam H‐fragment: lower frame (BHLF) RNA. and immunohistology (IH) for the identification of EBV‐encoded latent membrane protein 1 (LMP1) and of nuclear antigen (EBNA) 2 expression. EBV‐DNA was detectable by PCR in 15 of 76 cases (19·7%). EBER‐ISH combined with IH identified a variable, usually very low, number of infected neoplastic cells in only seven of the 15 EBV‐ONA‐harbouring cases. This discrepancy between the results obtained with PCR and ISH is apparently caused by the low number of the infected cells per tissue section. The PMTCL entity produced the greatest number of positive cases, whilst ALCL and LyP cases were almost constantly devoid of the virus. BHLF transcripts were not detectable in any case, nor did any of the EBER‐positive cells show an LMP1 or EBNA2 expression. These data show that primary cutaneous T‐cell lymphoproliferations display an infrequent association with a latent EBV infection and that the pathogenic role of the virus in the positive cases remains obscure as the virus frequently infects only a minority of the atypical cells.
评论：斯特拉顿讲座 1990。阳性和阴性造血干细胞调节剂的临床意义。
影响因子：
20.3
作者：
Moore,MA
通讯作者：
Moore,MA