Poly (ADP-ribosylation) and DNA topoisomerase I in different cell lines.
Poly (ADP-ribosylation) and DNA topoisomerase I in different cell lines.
复制标题
不同细胞系中的多聚（ADP-核糖基化）和 DNA 拓扑异构酶 I。
DOI：
10.1007/978-1-4684-8730-5_45
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发表时间：
1984
影响因子：
--
通讯作者：
Olivera,BM
中科院分区：
文献类型：
--
作者：
Ferro,AM;Thompson,LH;Olivera,BM
随着他发现大肠杆菌染色体是圆形的，约翰凯恩斯首先指出了DNA复制中旋转的必要性（1）。在真核生物中，I型DNA拓扑异构酶具有作为这种旋转所必需的酶性质。DNA复制将在复制叉之前引入正超扭曲; DNA拓扑异构酶I具有解开这种正超扭曲DNA的能力（2，3）。出于这个原因，拓扑异构酶I在复制中的作用已被许多工作者提出（2，3）。最近的生物学和现象学数据也表明I型DNA拓扑异构酶可能在某些条件下参与催化DNA链交换（4）。这样一种潜在的重要酶的活性可以被调节的机制是明显的兴趣。
With his discovery that theEscherichia colichromosome was circular, John Cairns first pointed out the need for a swivel in DNA replication (1). In eukaryotes, type I DNA topoisomerase has the enzymatic properties necessary to serve as such a swivel. DNA replication would introduce positive supertwists ahead of the replication fork; DNA topoisomerase I has the capacity to unwind such positively supertwisted DNA (2, 3). For this reason, a role for topoisomerase I in replication has been suggested by many workers (2, 3). Recent biological and phenomenological data have also led to the suggestion that type I DNA topoisomerase may be involved in catalyzing DNA strand exchange under certain conditions (4). The mechanisms by which the activity of such a potentially important enzyme may be modulated are of obvious interest.