Elevated mature macrophage expression of human ABHD2 gene in vulnerable plaque
Elevated mature macrophage expression of human ABHD2 gene in vulnerable plaque
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DOI：
10.1016/j.bbrc.2007.10.127
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发表时间：
2008-01-11
影响因子：
3.1
通讯作者：
Yamamura, Ken-Ichi
中科院分区：
文献类型：
--
作者：
Miyata, Kelshi;Nakayama, Masafumi;Yamamura, Ken-Ichi
我们先前报道了小鼠α/β水解酶结构域2（Abhd 2）在平滑肌细胞（SMC）中表达，抑制其迁移并通过袖带放置抑制内膜增生的发展;然而，ABHD 2在人体中的作用仍有待阐明。在这项研究中，我们检测了ABHD 2在不稳定型心绞痛（UA）和稳定型心绞痛（SA）患者冠状动脉粥样硬化病变中的表达。我们的研究结果表明，ABHD 2在动脉粥样硬化病变中表达，并且在UA患者中ABHD 2的表达显著高于SA患者。免疫组化显示ABHD 2在巨噬细胞中表达丰富，而在动脉粥样硬化病变的平滑肌细胞中表达较低。利用人血管原代培养细胞系，我们还证明ABHD 2在巨噬细胞中的表达显著高于SMC，并且ABHD 2的表达随着单核细胞向巨噬细胞的分化而显著成比例地增加。(c)2007爱思唯尔公司All rights reserved.
We previously reported that the mouse alpha/beta hydrolase domain containing 2 (Abhd2) was expressed in smooth muscle cells (SMCs) which suppressed their migration and inhibited the development of intimal hyperplasia by cuff placement; however, the role of ABHD2 in human remains to be elucidated. In this study, we examined ABHD2 expression in the human coronary atherosclerotic lesions of the patients with unstable angina (UA) and stable angina (SA). Our results showed that the ABHD2 was expressed in atherosclerotic lesions, and that the ABHD2 expression was significantly higher in the patients with UA than with SA. Immunohistochemistry analysis revealed abundant expression of ABHD2 in macrophages, but low expression in SMCs of atherosclerotic lesions. Using human vascular primary culture cell lines, we also demonstrated that the expression of ABHD2 was significantly higher in macrophages than in SMCs, and that the expression of ABHD2 significantly increased proportionally with differentiation from monocyte into macrophage. (c) 2007 Elsevier Inc. All rights reserved.