Localization of proliferating cell nuclear antigen in aural cholesteatoma.
Localization of proliferating cell nuclear antigen in aural cholesteatoma.
复制标题
耳胆脂瘤中增殖细胞核抗原的定位。
DOI：
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
M. Hirano
中科院分区：
文献类型：
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作者：
N. Uchida;S. Ito;M. Hirano
中耳胆脂瘤不是真正的肿瘤，但具有显着的增殖活性，导致乳突骨的严重破坏。在本研究中，我们用免疫组织化学方法结合抗增殖细胞核抗原抗体对胆脂瘤组织中增殖细胞核抗原的定位进行了研究，因为据说增殖细胞核抗原是一种非常有用的显示细胞增殖活性的蛋白质。在8例手术标本中，3例胆脂瘤上皮生发基底层细胞呈中等浓度的增殖细胞核抗原表达。此外，在1例非常活跃的溶骨性胆脂瘤中，与破坏的乳突骨病变直接接触的间充质细胞(可能是成纤维细胞样细胞)显示有增殖细胞核抗原活性。虽然中耳胆脂瘤组织的侵袭和增殖活性的病因和组织病理学尚不清楚，但这一观察结果表明，用增殖细胞核抗原抗体进行免疫组织病理学检查可能是评估骨吸收活性和判断各种类型胆脂瘤预后的有用工具。
Middle ear cholesteatoma is not a genuine tumor but has a remarkable proliferative activity which causes serious destruction of the mastoid bone. In the present study, we used immunohistochemistry with antiproliferating cell nuclear antigen (PCNA) antibody on cholesteatomatous tissues to evaluate the localization of PCNA, as it has been said that PCNA is a very available protein for showing cell proliferative activity. Moderately concentrated PCNA was demonstrated within the germinal basal layer cells of the cholesteatomatous epithelium in three of eight surgical specimens. Furthermore, in one case of very active osteolytic cholesteatoma, PCNA activity was demonstrated in the mesenchymal cells, probably fibroblast-like cells, in direct contact to the destroying mastoid bone lesions. Although the etiology and histopathology of the invasive and proliferative activity of middle ear cholesteatomatous tissues are unclear, this observation suggests that immunohistopathological examination using PCNA antibody might be a useful tool for evaluating bone resorption activity and for establishing the prognosis of various types of cholesteatoma.
DOI：
10.1016/s0021-9258(18)80079-5
发表时间：
1989-08
期刊：
The Journal of biological chemistry
影响因子：
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作者：
G. Morris;M. Mathews
通讯作者：
G. Morris;M. Mathews