Sympathomimetic drugs stimulate the output of secretory glycoproteins from human bronchi in vitro.
Sympathomimetic drugs stimulate the output of secretory glycoproteins from human bronchi in vitro.
复制标题
拟交感神经药物在体外刺激人支气管分泌糖蛋白的输出。
作者：
R. Phipps;Isobel P. Williams;P. Richardson;J. Pell;R. Pack;N. Wright
1.我们描述了一种方法，用于支持件的人支气管在Ussing室，放射性标记的内容物的分泌细胞与35 S，并收集放射性标记的大分子分泌到管腔方面的组织。这种方法以前曾用于研究动物的气道分泌物[R. J. Phipps，J. A.纳德尔和B。Davis，American Review of Respiratory Disease，（1980）121，359-365]。证据表明，放射性标记的分子是分泌性糖蛋白，可能是粘液糖蛋白。2.苯肾上腺素，一种α-肾上腺素受体激动剂，增加了支气管分泌放射性标记糖蛋白的速率。α-肾上腺素能受体拮抗剂胸腺嘧啶阻断这种作用，但β-肾上腺素能受体拮抗剂普萘洛尔没有。3.多巴酚丁胺，一种β-肾上腺素受体激动剂，增加了放射性标记的糖蛋白的分泌速率。普萘洛尔阻断了这一作用，但胸腺素没有。4.沙丁胺醇，β 2-肾上腺素受体激动剂，也增加了放射性标记的糖蛋白的分泌速率。普萘洛尔阻断了这种作用。5.我们的结论是，α-和β-肾上腺素受体激动剂在体外增加人支气管中糖蛋白分泌的速率，这几乎肯定意味着它们增加粘液分泌的速率。
1. We describe a method for supporting pieces of human bronchi in Ussing chambers, for radiolabelling the contents of the secretory cells with 35S, and for collecting radiolabelled macromolecules secreted on to the luminal aspect of the tissue. This method has previously been used to study airway secretions in animals [R. J. Phipps, J. A. Nadel & B. Davis, American Review of Respiratory Disease, (1980) 121, 359-365]. Evidence is given that the radiolabelled molecules are secretory glycoproteins, probably mucus glycoproteins. 2. Phenylephrine, an alpha-adrenoceptor agonist, increased the rate at which the bronchi secreted radiolabelled glycoproteins. Thymoxamine, an alpha-adrenoceptor antagonist, blocked this effect but propranolol, a beta-adrenoceptor antagonist, did not. 3. Dobutamine, a beta-adrenoceptor agonist, increased the rate of secretion of radiolabelled glycoproteins. Propranolol blocked this but thymoxamine did not. 4. Salbutamol, a beta 2-adrenoceptor agonist, also increased the rate of secretion of radiolabelled glycoproteins. Propranolol blocked this effect. 5. We conclude that both alpha- and beta- adrenoceptor agonists increase the rate of glycoprotein secretion in human bronchi in vitro and that this almost certainly means that they increase the rate of mucus secretion.