TRACHEOBRONCHIAL EPITHELIUM OF THE SHEEP .1. QUANTITATIVE LIGHT-MICROSCOPIC STUDY OF EPITHELIAL-CELL ABUNDANCE, AND DISTRIBUTION
TRACHEOBRONCHIAL EPITHELIUM OF THE SHEEP .1. QUANTITATIVE LIGHT-MICROSCOPIC STUDY OF EPITHELIAL-CELL ABUNDANCE, AND DISTRIBUTION
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DOI：
10.1002/ar.1092050304
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发表时间：
1983-01-01
影响因子：
--
通讯作者：
PLOPPER, CG
中科院分区：
文献类型：
--
作者：
MARIASSY, AT;PLOPPER, CG
5只去势羊经戊二醛灌注后，经左颅叶和尾叶轴向气道显微解剖。气道分支被分配二位数，表明它们在气管支气管树中的特定位置。已知气道生成的样品用树脂包埋，并用光镜检查。根据细胞形态、染色性质和分布的差异，识别出8个主要细胞组并定量如下：4类黏液细胞（M1、M2、M3和M4）、纤毛细胞、基底细胞、Clara细胞和浆液细胞。最后一类细胞仅限于粘膜下腺。气管上皮每单位长度的细胞最多，主要是由于大量的基底细胞。没有软骨和腺体的气道上皮中有基底细胞。近端气道粘膜细胞总数（M1、M2和M3）相对稳定。M4黏液细胞存在于近端气道腺体和无腺体气道上皮内。最远端气道由克拉拉细胞和纤毛细胞排列。少数最近端的非软骨气道有粘液细胞（M1、M2、M3和M4）、基底细胞和Clara细胞共享上皮衬里。在绵羊肺中，上皮细胞分布与气道壁成分无关；同一气道内壁可有1种以上的分泌性上皮细胞；Clara细胞的分布是基于气道生成和靠近肺泡。
Glutaraldehyde-infused tracheas and airways of 5 castrated sheep were microdissected following the axial airway of the left cranial and caudal lobes. Airway branches were assigned binary numbers indicating their specific location in the tracheobronchial tree. Samples of known airway generation were resin embedded and examied by light microscopy. Based on differences in cell morphology, staining properties and distribution, 8 major cell groups were recognized and quantified as follows: 4 mucous cell categories (M1, M2, M3 and M4), ciliated, basal, Clara and serous cells. The last cell category was restricted to submucosal glands. Tracheal epithelium had the most cells per unit length, primarily due to large numbers of basal cells. Basal cells are found in the epithelium of airways without cartilage or glands. The total mucous cell population (M1, M2 and M3) in proximal airways was relatively constant. M4 mucous cells were present in glands of proximal airways and in the epithelial lining of the airways without glands. The most distal airways were lined by Clara and ciliated cells. A small number of the most proximal noncartilaginous airways had mucous (M1, M2, M3 and M4), basal and Clara cells sharing the epithelial lining. In the sheep lung, epithelial cell distribution does not correlate with airway wall components; more than 1 type of secretory epithelial cell can share the lining of the same airway; and Clara cell distribution is based on airway generation and proximity to alveoli.