Construction and characterization of the hybrid bacteriophage lambda Charon vectors for DNA cloning
Construction and characterization of the hybrid bacteriophage lambda Charon vectors for DNA cloning
复制标题
用于 DNA 克隆的杂交噬菌体 lambda Charon 载体的构建和表征
DOI：
10.1128/jvi.29.2.555-575.1979
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发表时间：
1979
影响因子：
5.4
通讯作者：
F. Blattner
中科院分区：
文献类型：
--
作者：
B. Williams;F. Blattner
已构建了20个专为分子克隆而设计的杂交λ-κ，并命名为Charon λ-κ。通过可用于分离和检测克隆片段的掺入的选择和筛选，通过可用于控制克隆片段的转录的方式，通过可用于克隆的不同限制酶，通过可用于控制复制和转录的噬菌体免疫，以及它们所包含的生物安全特性。用于生产的载体的交叉进行了描述，并讨论了他们的家谱。每个载体的结构已通过遗传试验、DNA长度测定、DNA异源双链体的电子显微镜分析和限制性内切酶的凝胶电泳进行了验证。在这些结构的过程中，一个新的EcoRI位点被发现在一个衍生的lambda Aam 32 Bam 1的地图非常接近左内聚端的lambda。
Twenty hybrid lambda phages especially designed for molecular cloning have been constructed and named Charon phages. These phages differ in the ranges of sizes of DNA fragments that may be inserted, by the selections and screens which may be used to isolate and detect the incorporation of cloned fragments, by the way transcription of the cloned fragment may be controlled, by the different restriction enzymes that can be used for cloning, by the phage immunities that may be employed for controlling replication and transcription, and by the biological safety features that they contain. The crosses used to produce the vectors are described, and their genealogy is discussed. The structure of each vector has been verified by genetic tests, by DNA length determinations, by electron micrographic analysis of DNA heteroduplexes, and by gel electrophoresis of restriction enzyme digests. In the course of these constructions, a new EcoRI site was found in a derivative of lambda Aam32Bam1 which maps very near the left cohesive end of lambda.