Primary CXCR4 Co-receptor Use in Acute HIV Infection Leads to Rapid Disease Progression in the AE Subtype
Primary CXCR4 Co-receptor Use in Acute HIV Infection Leads to Rapid Disease Progression in the AE Subtype
复制标题
主要 CXCR4 辅助受体在急性 HIV 感染中的使用导致 AE 亚型疾病快速进展
DOI：
10.1089/vim.2012.0035
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发表时间：
2012-08-01
期刊：
影响因子：
2.2
通讯作者：
Wu, Hao
中科院分区：
文献类型：
--
作者：
Jiao, Yanmei;Song, Yingxue;Wu, Hao
这是一项比较研究，在HIV感染的早期阶段，两个不同的患者组，一个是低CD4计数（组1），另一个是高CD4计数（组2）。1组在2 y内CD4细胞计数低于200细胞/ μ L， 2组在2 y内CD4细胞计数高于500细胞/ μ L。从这些患者的血浆中提取病毒RNA，克隆并测序HIV-1 env基因的C2-V5区。使用Geno2pheno和PSSM程序基于V3环氨基酸序列预测共受体的使用。我们的研究结果表明，在快速进展的急性HIV感染（低CD4计数，组1）中，主要的共受体使用是CXCR4，而在高CD4计数组（组2）中，主要的共受体使用是CCR5。这些患者一年的随访数据显示，两组患者的HIV共受体使用均无明显变化。两组患者的序列分析显示AE亚型的流行，因此我们可以推测CXCR4共受体可能是HIV-1 AE亚型中使用的主要HIV-1共受体，并且可能是导致MSM队列中HIV-1疾病快速进展的原因。
This is a comparative study of HIV co-receptor usage in the early stages of HIV infection between two distinct patient groups, one with a low CD4 count (group 1), and the other with a high CD4 count (group 2). Group 1 progressed to a CD4 count below 200 cells/mu L within 2 y, while group 2 had a CD4 count above 500 cells/mu L within 2 y. Viral RNA was extracted from the plasma of these patients, and the C2-V5 region of the HIV-1 env genes were cloned and sequenced. The co-receptor usage was predicated based on V3 loop amino acid sequences using Geno2pheno and PSSM programs. Our results indicate that in acute HIV infection of rapid progressors (low CD4 count; group 1), the primary co-receptor usage is CXCR4, while in the high CD4 count group (group 2), the co-receptor usage is predominantly CCR5. One-year follow-up data from these patients showed no obvious change in HIV co-receptor usage in either group. Sequence analysis of patients from both study groups showed prevalence of the AE subtype, and therefore we can speculate that the CXCR4 co-receptor may be the primary HIV-1 co-receptor used in the HIV-1 AE subtype, and may be responsible for rapid HIV-1 disease progression in the MSM cohort.