Spinal Shox2 interneuron interconnectivity related to function and development
Spinal Shox2 interneuron interconnectivity related to function and development
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DOI：
10.7554/elife.42519
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发表时间：
2018-12-31
期刊：
影响因子：
7.7
通讯作者：
Dougherty, Kimberly J.
中科院分区：
文献类型：
--
作者：
Ha, Ngoc T.;Dougherty, Kimberly J.
产生后肢运动的神经元网络位于脊髓中。脊髓节律发生的潜在机制尚不清楚，但兴奋性中间神经元的网络活动和相互连接可能发挥重要作用。在这里，我们调查内的Shox2 interneuron人口，其中一个子集已被建议参与运动节律的产生，使用成对的记录在离体脊髓或切片转基因小鼠的互联性。稀疏的单向连接与化学突触传递和突出的双向连接介导的电突触内不同的子集的Shox2 interneurons。此外，双向电连接之间的功能相关的Shox2的中间神经元被优先发现。虽然在新生小鼠中普遍存在，但在3周龄的小鼠中，电耦合的发生率和强度开始下降。总体而言，我们的数据表明，间隙连接耦合促进Shox2中间神经元的同步化，并可能与发育中小鼠的运动节律有关。
Neuronal networks generating hindlimb locomotion are located in the spinal cord. The mechanisms underlying spinal rhythmogenesis are unknown but network activity and interconnectivity of excitatory interneurons likely play prominent roles. Here, we investigate interconnectivity within the Shox2 interneuron population, a subset of which has been suggested to be involved in locomotor rhythm generation, using paired recordings in isolated spinal cords or slices from transgenic mice. Sparse unidirectional connections consistent with chemical synaptic transmission and prominent bidirectional connections mediated by electrical synapses were present within distinct subsets of Shox2 interneurons. Moreover, bidirectional electrical connections were preferentially found between functionally-related Shox2 interneurons. Though prevalent in neonatal mice, electrical coupling began to decline in incidence and strength in mice similar to 3 weeks of age. Overall, our data suggest that gap junctional coupling promotes synchronization of Shox2 interneurons, and may be implicated in locomotor rhythmicity in developing mice.