X-ray crystallographic analysis of measles virus hemagglutinin.
X-ray crystallographic analysis of measles virus hemagglutinin.
复制标题
麻疹病毒血凝素的 X 射线晶体分析。
DOI：
10.2222/jsv.58.1
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Y. Yanagi
中科院分区：
文献类型：
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作者：
T. Hashiguchi;K. Maenaka;Y. Yanagi
X射线晶体学分析与核磁共振一起揭示了许多重要病毒蛋白质的三维结构，从而使我们能够更好地了解病毒和宿主细胞分子之间的相互作用。在这篇综述中，我们总结了最近确定的麻疹病毒（MV）附着蛋白血凝素的晶体结构。基于这种结构信息，我们还讨论了MV血凝素如何与各种细胞受体相互作用，以及为什么MV疫苗多年来一直有效而不诱导逃逸突变病毒。讨论的其他主题是一个假定的MV受体存在于极化上皮细胞和蛋白质表达系统，使用培养的人细胞系293 SGnTI（-），这是适合于X射线晶体学分析。
X-ray crystallographic analyses, together with nuclear magnetic resonance, have revealed three-dimensional structures of many important viral proteins, thereby allowing us to better understand the interactions between viral and host cell molecules. In this review, we summarize the recently determined crystal structure of the measles virus (MV) attachment protein hemagglutinin. Based on this structural information, we also discuss how the MV hemagglutinin interacts with various cellular receptors and why MV vaccines have been effective for many years without inducing escape mutant viruses. Other topics discussed are a putative MV receptor present on polarized epithelial cells and the protein expression system using a cultured human cell line 293SGnTI(-), which is suitable for X-ray crystallographic analyses.