Microfluidic genetic analysis with an integrated a-Si:H detector.
Microfluidic genetic analysis with an integrated a-Si:H detector.
复制标题
使用集成 a-Si:H 探测器进行微流控遗传分析。
DOI：
10.1007/s10544-005-1595-y
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Mathies,RA
中科院分区：
文献类型：
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作者：
Kamei,T;Toriello,NM;Lagally,ET;Blazej,RG;Scherer,JR;Street,RA;Mathies,RA
We have developed an integrated hydrogenated amorphous silicon (a-Si:H) fluorescence detector for microfluidic genetic analysis. It consists of a half-ball lens, a ZnS/YF3multilayer optical interference filter with a pinhole, and an annular a-Si:H PIN photodiode allowing the laser excitation to pass up through the central aperture in the photodiode and the filter. Microfluidic separations of multiplex PCR products generated from methicillin-resistant/sensitiveStaphylococcus aureus(MRSA/MSSA) DNA on microfluidic capillary electrophoresis (CE) devices are successfully detected with the integrated detector. Similarly, multiplex PCR amplicons from the kanamycin resistant and K12 serotype-specific genes ofE. colicells are detected. The direct detection of multiplex PCR amplicons indicates that the fluorescence detector can be successfully coupled with current microfluidic PCR-CE platforms. This work establishes that the integrated a-Si:H detector provides relevant limits of detection for point-of-care genetic and pathogen analysis with microfluidic devices.
