α6-Integrin Subunit Plays a Major Role in the Proangiogenic Properties of Endothelial Progenitor Cells
α6-Integrin Subunit Plays a Major Role in the Proangiogenic Properties of Endothelial Progenitor Cells
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DOI：
10.1161/atvbaha.110.209163
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发表时间：
2010-08-01
影响因子：
8.7
通讯作者：
Helley, Dominique
中科院分区：
文献类型：
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作者：
Bouvard, Claire;Gafsou, Benjamin;Helley, Dominique
α-α 6整联蛋白亚基（α 6）表达通过促血管生成生长因子如血管内皮生长因子（VEGF）和成纤维细胞生长因子增加。这种增加与内皮细胞及其称为内皮集落形成细胞（ECFC）的祖细胞的体外管形成增强相关。因此，我们研究了α 6的作用，在小鼠模型的后肢ischemia.Methods和Results-We使用小干扰RNA（siRNA）抑制α 6的ECFCs的表面上的表达诱导的人ECFCs的血管生成。对于体内研究，将人ECFC静脉注射到单侧后肢缺血的裸小鼠模型中。siRNA α 6转染可抑制ECFC诱导的缺血后肢新生血管形成和再灌注（P
Objective-Alpha6 integrin subunit (alpha 6) expression is increased by proangiogenic growth factors such as vascular endothelial growth factor (VEGF) and fibroblast growth factor. This increase correlates with enhanced in vitro tube formation by endothelial cells and their progenitors called Endothelial Colony-Forming Cells (ECFCs). We thus studied the role of alpha 6 in vasculogenesis induced by human ECFCs, in a mouse model of hindlimb ischemia.Methods and Results-We used small interfering RNA (siRNA) to inhibit alpha 6 expression on the surface of ECFCs. For in vivo studies, human ECFCs were injected intravenously into a nude mouse model of unilateral hind limb ischemia. Transfection with siRNA alpha 6 abrogated neovessel formation and reperfusion of the ischemic hind limb induced by ECFCs (P