Biofilm rupture by laser-induced stress waves increases with loading amplitude, independent of location
Biofilm rupture by laser-induced stress waves increases with loading amplitude, independent of location
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DOI：
10.1101/827964
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发表时间：
2019-11
期刊：
影响因子：
--
通讯作者：
K. L. Kearns;James D. Boyd;Martha E. Grady
中科院分区：
文献类型：
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作者：
K. L. Kearns;James D. Boyd;Martha E. Grady
安全和生物相容性医疗器械的生产不可或缺的是确定影响或控制强生物膜粘附的界面特性。激光溅射技术最近已经成为一种有利的方法来量化生物膜粘附在候选生物医学表面。然而，有可能膜张力是有助于分离生物膜和基质所需的应力的因素。在这种情况下，由激光能量密度控制的启动生物膜破裂的应力幅度将随着生物膜上的位置而系统地变化。当薄膜材料在应力波加载期间被弹出时，薄膜破裂也被称为破裂。为了确定膜张力的影响，我们提出了一个协议，测量剥落的大小与增加激光能量密度（可变能量密度）和相对于距离生物膜质心（等能量密度）。变形链球菌生物膜钛基板作为我们的模型系统。在这项研究中，共185个生物膜负载位置进行了分析。我们表明，生物膜剥落的大小单调增加激光能量密度和我们的程序失败的非生物膜。在iso-fluence实验中，没有发现生物膜剥落的大小和加载位置之间的相关性，从而提供了证据，膜张力不起主导作用，在生物膜粘附测量。我们建议我们的程序作为一个简单的方法来确定膜的影响，通过激光spectrometry技术在基板表面上的生物膜的附着力的测量。图形摘要
Integral to the production of safe and biocompatible medical devices is to determine the interfacial properties that affect or control strong biofilm adhesion. The laser spallation technique has recently emerged as an advantageous method to quantify biofilm adhesion across candidate biomedical surfaces. However, there is a possibility that membrane tension is a factor that contributes to the stress required to separate biofilm and substrate. In that case, the stress amplitude, controlled by laser fluence, that initiates biofilm rupture would vary systematically with location on the biofilm. Film rupture, also known as spallation, occurs when film material is ejected during stress wave loading. In order to determine effects of membrane tension, we present a protocol that measures spall size with increasing laser fluence (variable fluence) and with respect to distance from the biofilm centroid (iso-fluence). Streptococcus mutans biofilms on titanium substrates serves as our model system. A total of 185 biofilm loading locations are analyzed in this study. We demonstrate that biofilm spall size increases monotonically with laser fluence and apply our procedure to failure of non-biological films. In iso-fluence experiments, no correlation is found between biofilm spall size and loading location, thus providing evidence that membrane tension does not play a dominant role in biofilm adhesion measurements. We recommend our procedure as a straightforward method to determine membrane effects in the measurement of adhesion of biological films on substrate surfaces via the laser spallation technique. Graphical Abstract